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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,876 results for: sgrna

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Showing: 1721 - 1760 of 8876 results

  4. pWS082

    Plasmid
    #90516
    Purpose
    sgRNA entry vector
    Depositor
    Tom Ellis
    Insert
    Yeast sgRNA expression vector
    Use
    CRISPR; Yeast gap repair
    Expression
    Yeast
    Available Since
    Oct. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    90516-plasmid-map-sequence-id-197186

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  8. pBHM2646

    Plasmid
    #229530
    Purpose
    Carries mAID_opt tag, empty sgRNA, and NATR marker
    Depositor
    Hiten Madhani
    Insert
    mAID_opt - empty sgRNA - NATR
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229530-plasmid-map-sequence-id-459934

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  9. lentiCRISPRv2_ZsG gRNA_4

    Plasmid
    #192688
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #4
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    192688-plasmid-map-sequence-id-379587

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  10. lentiCRISPRv2_ZsG gRNA_1

    Plasmid
    #192685
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192685-plasmid-map-sequence-id-379591

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  11. lentiCRISPRv2_ZsG gRNA_2

    Plasmid
    #192686
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192686-plasmid-map-sequence-id-379590

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  12. lentiCRISPRv2_ZsG gRNA_3

    Plasmid
    #192687
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192687-plasmid-map-sequence-id-379588

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  17. PX458 V3

    Plasmid
    #226957
    Purpose
    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226957-plasmid-map-sequence-id-450777

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  19. pAAV_ACTB CRISPIE

    Plasmid
    #172853
    Purpose
    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Inserts
    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172853-plasmid-map-sequence-id-341002

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  20. pBHM2644

    Plasmid
    #229528
    Purpose
    Carries AID* tag, empty sgRNA, and NATR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - NATR
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229528-plasmid-map-sequence-id-459927

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  21. pBHM2645

    Plasmid
    #229529
    Purpose
    Carries mIAA7_opt tag, empty sgRNA, and NATR marker
    Depositor
    Hiten Madhani
    Insert
    mIAA7_opt - empty sgRNA - NATR
    Use
    CRISPR
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229529-plasmid-map-sequence-id-459936

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  22. pBHM2650

    Plasmid
    #229992
    Purpose
    Carries AID* tag, empty sgRNA, and HYGR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - HYGR
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229992-plasmid-map-sequence-id-461779

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  23. pBHM2652

    Plasmid
    #229994
    Purpose
    Carries AID* tag, empty sgRNA, and amdS marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - amdS
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229994-plasmid-map-sequence-id-461120

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  24. pDECKO_GFP_AAVS1_186

    Plasmid
    #159091
    Purpose
    targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    159091-plasmid-map-sequence-id-308784

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  25. pDECKO_mCherry_AAVS1

    Plasmid
    #141212
    Purpose
    pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141212-plasmid-map-sequence-id-299605

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  26. gRNA telo/GFP

    Plasmid
    #137713
    Purpose
    Vector co-expressing sgRNA telo and GFP
    Depositor
    Dominik Niopek
    Insert
    sgRNA telo + GFP
    Use
    AAV, CRISPR, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6/RSV
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    137713-plasmid-map-sequence-id-272438

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  27. pMZ374

    Plasmid
    #59896
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available Since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59896-plasmid-map-sequence-id-313658

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  32. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid
    #208647
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208647-plasmid-map-sequence-id-418374

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  33. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid
    #208979
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208979-plasmid-map-sequence-id-418375

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  39. pBHM2651

    Plasmid
    #229993
    Purpose
    Carries AID* tag, empty sgRNA, and NEOR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - NEOR
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229993-plasmid-map-sequence-id-461132

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Showing: 1721 - 1760 of 8876 results