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Plasmid#121463Purpose2µm HIS3 CNB1mutEF1DepositorInsertCNB1mutEF1 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 34 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK516
Plasmid#121473PurposeCEN LEU2 CNB1mutEF4DepositorInsertCNB1mutEF4 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 144 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK506
Plasmid#121461PurposeCEN HIS3 CNB1mutEF4DepositorInsertCNB1mutEF4 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 144 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK505
Plasmid#121460PurposeCEN HIS3 CNB1mutEF3DepositorInsertCNB1mutEF2 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 103 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK504
Plasmid#121459PurposeCEN HIS3 CNB1mutEF2DepositorInsertCNB1mutEF2 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 66 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK503
Plasmid#121458PurposeCEN HIS3 CNB1mutEF1DepositorInsertCNB1mutEF1 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 34 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(F317D, I325A)
Plasmid#122275PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317D, I325A)
Plasmid#122273PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317D)
Plasmid#121391PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterADH1 promoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(L105D)
Plasmid#121395PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationLeucine 105 to Aspartic acidPromoterADH1 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(I325D)
Plasmid#121397PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationIsoleucine 325 to Aspartic acidPromoterADH1 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(L331D)
Plasmid#121393PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationLeucine 331 to Aspartic acidPromoterADH1 promoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(L105D)
Plasmid#121388PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationLeucine 105 to Aspartic acidPromoterADH1 promoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(I325D)
Plasmid#121392PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationIsoleucine 325 to Aspartic acidPromoterADH1 promoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(I310D)
Plasmid#121389PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationIsoleucine 310 to Aspartic acidPromoterADH1 promoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(F317D)
Plasmid#121396PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterADH1 promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-3xFLAG-OB D166R
Plasmid#79061PurposeRetroviral expression of 3xFLAG tagged, nuclear localized TPP1-OB-RR folding domainDepositorInsertTPP1 (TPP1 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationAspartic acid 166 changed to ArgininePromoterCMVAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK381
Plasmid#71573PurposeProduces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)DepositorInsertcitrate synthase
UseTagsExpressionBacterialMutationchanges aspartate-317 to glycinePromoterrecAAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK347
Plasmid#71833PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationchanges histidine-59 to aspartatePromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK348
Plasmid#71832PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparaginePromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only