We narrowed to 17,876 results for: she

1. pOZC-PALB2

   Plasmid

   #133552

   Purpose

   PALB2 coding sequence in pOZ-FH-C C-terminal Flag, His tagging retroviral vector.

   Depositor

   David Livingston

   Article

   Xia et al Mol Cell. 2006 Jun 23. 22(6):719-29.

   Insert

   PALB2 (PALB2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMYOCWT-EGFP

   Plasmid

   #39326

   Depositor

   Beatrice Yue

   Article

   Koga et al Am J Pathol. 2010 Jan;176(1):343-52. Epu

   Insert

   Myocilin wild-type (MYOC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-blast shKGA

   Plasmid

   #110420

   Purpose

   shKGA, silence glutaminase KGA isoform, blasticidin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (RNA Pol III)

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Vector 1174D

   Plasmid

   #200012

   Purpose

   Fluorescent based sex separator in Aedes aegypti (SEPARATOR)

   Depositor

   Omar Akbari

   Article

   Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.

   Insert

   Engineered dobulesex splicing module
   

   Use

   Synthetic Biology

   Tags

   Expression

   Insect

   Mutation

   Engineered dobulesex splicing module

   Promoter

   Hr5IE1

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

   Plasmid

   #174091

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV and T7

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pG-HIV-LRT

   Plasmid

   #104592

   Purpose

   Standard for qPCR

   Depositor

   Kristine Yoder

   Article

   Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

   Insert

   HIV-1 late reverse transcripts
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMyc-CMV1

   Plasmid

   #53582

   Purpose

   mammalian expression vector with Myc tag

   Depositor

   Linda Yu

   Article

   Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-Kras G12V-T35S

   Plasmid

   #58900

   Purpose

   Expresses human Kras with G12V and T35S mutations

   Depositor

   Channing Der

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G12V and T35S mutations (effector binding mutant)

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

9. Hs.KRAS4b S17N

   Plasmid

   #83156

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   S17N

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hs.KRAS4b G12R

    Plasmid

    #83143

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12R

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-dCas9-p300-Hygro

    Plasmid

    #104411

    Purpose

    transient expression of dCas9-p300 fusion protein

    Depositor

    Stephan Beck

    Insert

    p300 (EP300 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. DOT1L

    Plasmid

    #36196

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (GeneScript Synthesized) (DOT1L Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-420

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

13. DOT1L

    Plasmid

    #40736

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. VPS13D^Halo

    Plasmid

    #174108

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    With an internal Halo tag in aminoacid position 1…

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-mito-CitroffRH

    Plasmid

    #158548

    Purpose

    Mitochondria-targeted nonbinding variant of inverse-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitroffRH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSA110

    Plasmid

    #59784

    Purpose

    drives mCherry in C. elegans intestinal cells

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    elt-2 promoter (elt-2 Nematode)
    mCherry (worm)
    unc-54 3' UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. SP6-sgRNA-scaffold

    Plasmid

    #47912

    Purpose

    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

    Depositor

    Paul Sternberg

    Article

    Chiu et al Genetics. 2013 Aug 26.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    sgRNA 3' end

    Expression

    Worm

    Mutation

    Promoter

    SP6

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-FLAG-Sestrin2-E451A

    Plasmid

    #73673

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E451A changed Glutamate 451 to Alanine

    Promoter

    CMV

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pLI_TNF

    Plasmid

    #171179

    Purpose

    inducible expression of human TNF

    Depositor

    Veit Hornung

    Article

    Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

    Insert

    human TNF
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMBP-LbCas12a D832A

    Plasmid

    #113517

    Purpose

    Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    LbCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    D832A

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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