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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,034 results for: Pol

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Showing: 8821 - 8840 of 10034 results

  6. pOCC154

    Plasmid
    #118902
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118902-plasmid-map-sequence-id-232325

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  9. pPLP2H-mCf

    Plasmid
    #229985
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229985-plasmid-map-sequence-id-458287

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  10. pPLP2S-mCf

    Plasmid
    #229986
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    Strep-tag II
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229986-plasmid-map-sequence-id-458306

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  13. pOCC153

    Plasmid
    #118901
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118901-plasmid-map-sequence-id-232312

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  14. pOCC7

    Plasmid
    #118876
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118876-plasmid-map-sequence-id-232237

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  15. DCAF1:PBC020-D05:C246337

    Plasmid
    #183974
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    DCAF1:PBC020-D05:C246337 (DCAF1 Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Promoter
    Polyhedrin
    Available Since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183974-plasmid-map-sequence-id-361427

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  16. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Promoter
    SP6
    Available Since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47911-plasmid-map-sequence-id-68528

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  18. RNF168-Halo HRD

    Plasmid
    #207080
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Expression
    Mammalian
    Promoter
    None
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207080-plasmid-map-sequence-id-432323

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  19. TAF1

    Plasmid
    #39118
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1 (TAF1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 8821 - 8840 of 10034 results