We narrowed to 11,426 results for: 110

1. pCMV-HA-mIFITM3-K24A

   Plasmid

   #58400

   Purpose

   Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells

   Depositor

   Jacob Yount, Howard Hang

   Article

   Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Lysine 24 to Alanine

   Promoter

   CMV IE

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pDEST myc MOV10 K530A

   Plasmid

   #51719

   Purpose

   plasmid expresses myc-tagged MOV10 K530A

   Depositor

   Markus Landthaler

   Article

   Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

   Insert

   MOV10 K530A (MOV10 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   K530A

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAtCas9-H840A_1

   Plasmid

   #91162

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_H840A (nickase)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   H840A

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MET FLAG103QDProCFPp303

   Plasmid

   #15591

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 103Q Delta Pro (HTT Human)
   

   Use

   Tags

   CFP and FLAG

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

5. p_3xFLAG-Rpp25

   Plasmid

   #87194

   Purpose

   Expression of ectopic 3xFLAG-Rpp25 construct from CMV promoter

   Depositor

   Thomas Cech

   Article

   Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

   Insert

   3xFLAG-Rpp25 (RPP25 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TAL2268

   Plasmid

   #36705

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FOXO3-TALEN_Left (Foxo3 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

7. TAL2269

   Plasmid

   #36706

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FOXO3-TALEN_Right (Foxo3 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

8. Non-targeting CRISPRi plasmid

   Plasmid

   #154891

   Purpose

   Human non-targeting CRISPRi gRNA

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Lalli et al Genome Res. 2020 Sep;30(9):1317-1331. do

   Insert

   Nontargeting gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMOD_A0802

   Plasmid

   #91023

   Purpose

   Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-AtCas9_dead (D10A + H840A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, H840A

   Promoter

   AtUbi10

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAtCas9-dead_1

    Plasmid

    #91165

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A+H840A (dead)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJG493

    Plasmid

    #91197

    Purpose

    WDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gUbi1+gUbi8+donor
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 815 pSG5L HA PTEN delta274-342

    Plasmid

    #10738

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 274-342.

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pDEST myc MOV10 D645N

    Plasmid

    #51720

    Purpose

    plasmid expresses myc-tagged MOV10 D645N

    Depositor

    Markus Landthaler

    Article

    Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

    Insert

    MOV10 D645N (MOV10 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D645N

    Promoter

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTaCas9_5

    Plasmid

    #91168

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAtCas9-H840A_2

    Plasmid

    #91163

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_H840A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMDC84-SEIPIN3-Ndelta

    Plasmid

    #96981

    Purpose

    Express N-terminal truncated Arabidopsis SEIPIN3 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN3 (AT2G34380 Mustard Weed)
    

    Use

    Tags

    6xHis and GFP

    Expression

    Plant

    Mutation

    A segment of 507 nucleotides are deleted from the…

    Promoter

    35S

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. GAL 25Q+ProCFPp303

    Plasmid

    #15574

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt25Q + Pro (HTT Human)
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pSBL_DK198

    Plasmid

    #156425

    Purpose

    Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (aa 42-275)
    

    Use

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    aa 42-275

    Promoter

    Polyhedrin

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-RAP1

    Plasmid

    #84525

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    none

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJG486

    Plasmid

    #91195

    Purpose

    WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nTaCas9_H840A+gUbi1+donor
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge