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Flag-FOXC2

Plasmid

#153115

Purpose

Expression of Flag tagged human FOXC2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXC2 (FOXC2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLOX-CW-CRE

Plasmid

#12238

Depositor

Didier Trono

Article

Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

Insert

CRE

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pBEN276

Plasmid

#69150

Purpose

Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

Depositor

Pierre Germon

Article

Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

Insert

luxCDABE

Use

Luciferase

Promoter

frr

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD2

Plasmid

#153117

Purpose

Expression of Flag tagged human FOXD2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD2 (FOXD2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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mApple-TfR-RUSH

Plasmid

#166906

Purpose

RUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-mApple

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mApple

Expression

Mammalian

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-FOXS1

Plasmid

#153152

Purpose

Expression of Flag tagged human FOXS1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXS1 (FOXS1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pTetOne-mCherry-cNLS

Plasmid

#138527

Purpose

Inducible expression of mCherry with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

mCherry

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE3Gs

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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PAX6 donor plasmid

Plasmid

#105239

Purpose

Knock in H2B-GFP into the human PAX6 locus

Depositor

Lorenz Studer

Article

Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

Insert

PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette

Use

TALEN

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro2-H2B-iRFP

Plasmid

#197352

Purpose

Encoding nuclear-targeted iRFP713.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

iRFP with nucleus-targeting sequence

Use

Lentiviral

Tags

H2B (human histone 2B)

Expression

Mammalian

Promoter

EF-1α

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pKIBra-P2A-mCherry-Puro-sfGFP

Plasmid

#115686

Purpose

Knock-in of P2A-mCherry at the mouse Brachyury (T) gene

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

P2A-mCherry-loxP-PGK-Puro-sfGFP-loxP

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-mWatermelon

Plasmid

#190625

Purpose

Bacterial expression of mWatermelon GFP

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

mWatermelon

Expression

Bacterial

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SeeSaw Reporter (SSR2.0)

Plasmid

#50393

Purpose

SSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.

Depositor

Pablo Huertas Sánchez

Article

Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

Insert

SSR2.0

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST14-Avitag-SpyCatcher002

Plasmid

#102828

Purpose

Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Avitag-SpyCatcher002

Tags

Avitag, GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ROCK1

Plasmid

#187296

Purpose

Express GFP ROCK1

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (a.k.a. 1110055K06Rik, Rock-I) (Rock1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR TDP43 WT YFP

Plasmid

#27470

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pSico

Plasmid

#11578

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2-sgControl

Plasmid

#230080

Purpose

Crispr knock out control

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

non-specific sequence control

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-hNINJ1-GFP

Plasmid

#208779

Purpose

Inducibly expresses human NINJ1 tagged with GFP

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (NINJ1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Mouse ZP3 promoter (JD#445)

Plasmid

#14634

Depositor

Jurrien Dean

Article

Rankin et al Development. 1998 Jul . 125(13):2415-24.

Insert

ZP3 promoter (Zp3 Mouse)

Expression

Bacterial

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pBI-EGFP-MnSOD

Plasmid

#16612

Depositor

Bert Vogelstein

Article

Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

Insert

MnSOD (SOD2 Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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