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pAAV hSyn FLEx-FRT hM3Dq-mCherry

Plasmid

#161575

Purpose

Expression of hM3Dq-mCherry in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM3Dq-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pXCX.CMV.CREB.WPRE

Plasmid

#38050

Depositor

James Uney

Article

Glover et al Neuroreport. 2004 May 19;15(7):1171-5.

Insert

CREB (Creb1 Rat)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX307-SARS-CoV-3CL

Plasmid

#160280

Purpose

Allows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenLox_U6 Nlgn3

Plasmid

#59359

Purpose

Lentiviral expression vector: Neuroligin 3 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

Depositor

Peter Scheiffele

Article

Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

Inserts

Neuroligin 3 shRNA (rat) (Nlgn3 Rat)
eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG and mouse U6

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

Plasmid

#161580

Purpose

Express Kir2.1 in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD

Plasmid

#170124

Purpose

Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD-NES

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a core promoter

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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CMV500 A-SREBP2

Plasmid

#33358

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Insert

SREBP2

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
GAL 103Q+ProCFPp303

Plasmid

#15575

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt103Q + Pro (HTT Human)

Tags

CFP

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRS416 Gal TDP43 Q331K

Plasmid

#27461

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
pRS416 Gal-RNQ1-YFP

Plasmid

#18685

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
CMV-Cx35-Halo-C-end

Plasmid

#71512

Purpose

Mammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.

Depositor

John O'Brien

Article

Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

Insert

Connexin 35

Tags

HaloTag

Expression

Mammalian

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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mCardinal-MCAK-C-7

Plasmid

#56168

Purpose

Localization: Microtubules/Kinesin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

MCAK (KIF2C Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLV-CMV-hXRCC1-mCherry-Hygro

Plasmid

#176532

Purpose

mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

Insert

X-Ray Repair Cross Complementing 1 (XRCC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-α-Syn-nYFP

Plasmid

#92203

Purpose

Retroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

SNCA (SNCA Human)

Use

Retroviral

Tags

YFP(1-155)

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Ai95(RCL-GCaMP6f) targeting vector

Plasmid

#61579

Purpose

Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

CAG-LSL-GCaMP6-fast

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD5 (OTU, aa 171-358)

Plasmid

#61415

Purpose

Expresses human OTUD5 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD5 (OTUD5 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-170 and aa 359-571.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX6P2-p53 (1-90) (72R)

Plasmid

#62081

Purpose

Expression of human p53 (TAD residues 1-90) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains TAD/PP residues 1-90, 72R

Promoter

tac

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only
pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

Plasmid

#169912

Purpose

Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

dCjCas9

Use

CRISPR

Tags

10XGCN4, 2xSV40NLS, and SV40NLS-HA

Expression

Mammalian

Mutation

D8A, H559A in cjCas9

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-dSpCas9-HF1

Plasmid

#92115

Purpose

Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9-HF1 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, N497A, R661A, Q695A, H840A, Q926A

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pReporter_7

Plasmid

#60567

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_7

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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Cas9m3-VP64

Plasmid

#47318

Purpose

Cas9m3 Activator

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m3-VP64

Use

CRISPR

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
Sa-SlugCas9

Plasmid

#163797

Purpose

Expresses Sa-SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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MoFGFR2b-ires neo

Plasmid

#86491

Purpose

mamalian cell expression of FGFR2b

Depositor

David Ornitz

Article

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR2 IIIb 13A4 (FGFR2 Human)

Expression

Mammalian

Promoter

Moloney murine leukemia virus LTR

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-beta-Actin

Plasmid

#37136

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human beta-actin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-alpha-Tubulin

Plasmid

#37137

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human alpha-tubulin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pX165-Sniper-Cas9

Plasmid

#140560

Purpose

Expresses Sniper Cas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

F539S M763I K890N

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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TmSir2

Plasmid

#25815

Depositor

Cynthia Wolberger

Article

Avalos et al Mol Cell 2005 Mar 18;17(6):855-68.

Insert

Tm Sir2

Expression

Bacterial

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
GST-TEV-FUS-1-373aa

Plasmid

#29631

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Tags

GST and TEV

Expression

Bacterial

Mutation

Contains FUS aa# 1-373

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
423Gal-FUS-YFP

Plasmid

#29615

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-myc-ASPM MTB

Plasmid

#27668

Depositor

Joseph Loturco

Article

Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

Insert

Abnormal spindle-like microcephaly associated (Aspm Rat)

Tags

GFP

Expression

Mammalian

Mutation

Contains amino‑terminal putative microtubule bind…

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only