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Plasmid#162585PurposeExpresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationLeucine 144 changed to Cysteine and Proline 221 c…PromoterpolyhderinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 A59P
Plasmid#166816PurposeGateway pDONR vector encoding NLRP1 with the patient mutation A59P (Herlin et al., 2020) (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationA59PPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Ub-NLRP1 L1215
Plasmid#166828PurposepDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsUbiquitinExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Ub-NLRP1 S1213
Plasmid#166827PurposepDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus (no stop)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsUbiquitinExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 T755N
Plasmid#166820PurposeGateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationT755NPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 R726W
Plasmid#166819PurposeGateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationR726WPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Ub-NLRP1 L1218
Plasmid#166829PurposepDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsUbiquitinExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 NtSPrbcS (GB2213)
Plasmid#160589PurposeB3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chainDepositorInsertNtSPrbcS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/HuR.S221D
Plasmid#110347PurposeDoxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG tagDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationS221D, P237SPromotergagAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/HuR.S221D
Plasmid#110355PurposeDoxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and mKO2 tagsDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAG and mKO2ExpressionMammalianMutationS221D, P237SPromotergagAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-10X-tRNA
Plasmid#70123PurposeGateway DONR vector with cassette for expression of 10 intronless S. cerevisiae tRNAsDepositorInsert10X-tRNA block
UseTagsExpressionMutationPromoterNone – SUP4 pol III promoter provided by insertAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
ADCK5 gRNA (BRDN0001162211)
Plasmid#77320Purpose3rd generation lentiviral gRNA plasmid targeting human ADCK5DepositorInsertADCK5 (ADCK5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLAU gRNA (BRDN0001162221)
Plasmid#77104Purpose3rd generation lentiviral gRNA plasmid targeting human PLAUDepositorInsertPLAU (PLAU Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TEX14 gRNA (BRDN0001146221)
Plasmid#76832Purpose3rd generation lentiviral gRNA plasmid targeting human TEX14DepositorInsertTEX14 (TEX14 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
UCKL1 gRNA (BRDN0001162217)
Plasmid#76346Purpose3rd generation lentiviral gRNA plasmid targeting human UCKL1DepositorInsertUCKL1 (UCKL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL4-no stop
Plasmid#67323PurposehArl4 without stop codon in Gateway Entry vectorDepositorInsertARL4 (ARL4A Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 105 A to T; silent mutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL5A-no stop
Plasmid#67325PurposehArl5a without stop codon in Gateway Entry vectorDepositorInsertARL5A (ARL5A Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 70 C to T; silent mutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL5B-no stop
Plasmid#67327PurposehArl5b without stop codon in Gateway Entry vectorDepositorInsertARL5B (ARL5B Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL4C-no stop
Plasmid#67331PurposehArl4c without stop codon in Gateway Entry vectorDepositorInsertARL7 (ARL4C Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL4D-no stop
Plasmid#67337PurposehArl4D without stop codon in Gateway Entry vectorDepositorInsertARL4D (ARL4D Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL14-no stop
Plasmid#67339PurposehArl14 without stop codon in Gateway Entry vectorDepositorInsertARL14 (ARL14 Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 172 C to T; aa L58FPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARF1-no stop
Plasmid#67307PurposehArf1 without stop codon in Gateway Entry vectorDepositorInsertARF1 (ARF1 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARF3-no stop
Plasmid#67309PurposehArf3 without stop codon in Gateway Entry vectorDepositorInsertARF3 (ARF3 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARF4-no stop
Plasmid#67311PurposehArf4 without stop codon in Gateway Entry vectorDepositorInsertARF4 (ARF4 Human)
UseGateway entry vectorTagsExpressionBacterialMutationV53A, L123P and M134I mutations were unintended P…PromoterAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARF6-no stop
Plasmid#67315PurposehArf6 without stop codon in Gateway Entry vectorDepositorInsertARF6 (ARF6 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
si:ch211-221n23.1_R (OZ604)
Plasmid#35244DepositorInsertZinc finger array targeting si:ch211-221n23.1 (si:ch211-221n23.1 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
si:ch211-221n23.1_L (OZ603)
Plasmid#35243DepositorInsertZinc finger array targeting si:ch211-221n23.1 (si:ch211-221n23.1 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRLCon183-221_850-1207
Plasmid#31449DepositorInsertHNF4A 3'UTR fragments (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRLCon183-221mut2_850-1207
Plasmid#31450DepositorInsertHNF4A 3'UTR fragments (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationmut2 in balancerPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10-G1237A
Plasmid#24733DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationG replaced with A at 1237 position resulting in G…PromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10-C1240T
Plasmid#24735DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationC replaced with T at position 1240 resulting in H…PromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10-G1238A
Plasmid#24738DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationG replaced with A at position 1238 resulting in G…PromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-S2215Y
Plasmid#69013Purposeactivating MTOR mutationDepositorInsertMTOR (MTOR Human)
UseTagsFLAGExpressionMammalianMutationS2215YPromoterCMVAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-S2215Y
Plasmid#26037DepositorInsertmammalian target of rapamycin-S2215Y (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationS2215YPromoterAvailable sinceSept. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TAF15 c1222t No Stop
Plasmid#84900PurposeDONOR vector for Gateway cloning of TAF15 c1222t No StopDepositorInsertTAF15 (TAF15 Human)
UseGateway donorTagsExpressionMutationc1222t No StopPromoternoneAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
p221z-CAS9p-TagRFP-t35s
Plasmid#118386PurposeEntry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p-TagRFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-WT-long-UBASH3A-Ntag
Plasmid#192098PurposeHuman wild-type long-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-WT-long-UBASH3A-Ctag
Plasmid#192097PurposeHuman wild-type long-form UBASH3A in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALPS puro miR30-L1221
Plasmid#101335Purposenegative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGTDepositorInsertshRNA targeting miR30
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
221 pCS 3MT DEST
Plasmid#13070DepositorInsert6x Myc Tag
UseTagsExpressionMutationPromoterAvailable sinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLKO-STAG2 shRNA 1221
Plasmid#31978DepositorInsertSTAG2 (STAG2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSO221 Phis-72 BirA GFP
Plasmid#45100DepositorInsertsUseTagsGFPExpressionWormMutationPromoterhis-72 and unc-119Available sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
p221-RV-dlx-dio-Tdtomato
Plasmid#185697PurposeRetroviral vector expression cre-dependent membrane tagged Tdtomato driven by the mDlx promoterDepositorInsertmembrane Tdtomato
UseCre/Lox and RetroviralTagsGap43 palmitoylationExpressionMutationPromotermDlxAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 hNLRP3-mNG-STOP
Plasmid#218644PurposeGateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)DepositorInsertNLRP3 (NLRP3 Human)
UseGateway entryTagsmNeonGreenExpressionMutationPromoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-TrpV1 [N221/17R]
Plasmid#177517PurposeMammalian Expression Plasmid of anti-TrpV1 (Rat). Derived from hybridoma N221/17.DepositorInsertanti-TrpV1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-TrpV1 [N221/12R]
Plasmid#188224PurposeMammalian Expression Plasmid of anti-TrpV1 (Rat). Derived from hybridoma N221/12DepositorInsertanti-TrpV1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-5p
Plasmid#103366PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-5p target (MIR221 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-MICU1-sh1-rORF
Plasmid#31764DepositorInsertMICU1 (MICU1 Human)
UsePdonr gatewayTagsNoneExpressionMutationRNAi-resistant ORF (rORF). 8 synonymous changes i…PromoterNoneAvailable sinceMay 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-OSF-TRIM5-21R
Plasmid#79063PurposeGateway entry vector for cloning and propagation in DH5alphaDepositorInsertOSF-TRIM5-21R
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-3p
Plasmid#103365PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-3p target (MIR221 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only