We narrowed to 2,403 results for: T2A

1. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

   Plasmid

   #97044

   Purpose

   Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet on

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEpicVector3mC_Puro-T2A-mCherry-ProtC-FLAG-AU1

   Plasmid

   #162117

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to ProtC-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   ProtC-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEpicVector3mC_Puro-T2A-mCherry-VSVg-FLAG-AU1

   Plasmid

   #162108

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-FLAG

   Plasmid

   #162084

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-ProtC-FLAG
   

   Use

   Lentiviral

   Tags

   VSVg-ProtC-FLAG

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-AU1

   Plasmid

   #162091

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-ProtC-AU1
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC-attB-burs-mCD8-EGFP-T2A-Gal4

   Plasmid

   #39463

   Depositor

   Benjamin White

   Article

   Diao et al Genetics. 2012 Mar;190(3):1139-44. Epub

   Insert

   burs-mCD8-EGFP-T2A-Gal4
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   bursalpha

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

   Plasmid

   #87828

   Purpose

   High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter, tetracycline operator

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Expression

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

   Plasmid

   #128749

   Purpose

   Expresses control gRNA

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

    Plasmid

    #78304

    Purpose

    Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-nlsLexA::GADfluw-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

    Plasmid

    #205549

    Purpose

    Express EPN24-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #135012

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Tags

    3x Flag, 3xFLAG (N terminal on insert), NLS, and …

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)

    Plasmid

    #194229

    Purpose

    Lentiviral expression vector expressing LCB1 SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-LCB1-SynNotch-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV ArcLightCo (Q239)-T2A-nls-mCherry

    Plasmid

    #85806

    Purpose

    Genetically encoded voltage sensor ArcLight- codon optimized

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    ArcLightCo-Q239
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    CMV

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 H2B-mIFP T2A Mpro (C145A)

    Plasmid

    #163080

    Purpose

    Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B

    Depositor

    Xiaokun Shu

    Insert

    Inactive Mpro mutant C145A and H2B-mIFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDropIn-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199195

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV anti-FITC-LaG16-Y186F-T2A-BFP

    Plasmid

    #194931

    Purpose

    LV construct encoding Y186F mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-CB1-HIF2a-GFP-T2A-Puro

    Plasmid

    #71708

    Purpose

    Lentiviral expression of HIF2A-GFP

    Depositor

    Eric Jonasch, Xiande Liu

    Article

    Liu et al Oncogene. 2015 May 7;34(19):2450-60. doi

    Insert

    HIF2A (EPAS1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-KS-attB2-SA(1)-T2A-LexA::QFAD-Hsp70

    Plasmid

    #62948

    Purpose

    Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-LexA::QFAD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102887

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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