We narrowed to 2,475 results for: T2A

1. pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)

   Plasmid

   #221329

   Purpose

   Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   FLAG-IRSp53 and HA-Cdc42(G12V) (Baiap2, Cdc42 Mouse)
   

   Use

   Retroviral

   Mutation

   G12V

   Promoter

   TRE3G

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

   Plasmid

   #69534

   Purpose

   DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   DD-dCas9VP192-T2A-EGFP
   

   Tags

   C-term T2A-EGFP and DHFR Destabilised Domain

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

   Plasmid

   #141094

   Purpose

   Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

   Depositor

   Carolyn McBride

   Article

   Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

   Insert

   bruchpilot
   

   Use

   CRISPR

   Expression

   Insect

   Mutation

   coding sequence downstream of CRISPR cut site, sy…

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SOX9-T2A-NLS-tdTomato-PGK-Puro

   Plasmid

   #89993

   Purpose

   Donor template for generation of SOX9-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-AU1

   Plasmid

   #162111

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to StrepTagII-ProtC-AU1
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-AU1

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-FLAG

   Plasmid

   #162090

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-ProtC-FLAG
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-FLAG

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEpicVector3G_Puro-T2A-EGFP-ProtC-FLAG-AU1

   Plasmid

   #162097

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to ProtC-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   ProtC-FLAG-AU1

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-AU1

   Plasmid

   #162082

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-StrepTagII-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-AU1

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti spCas9 T2A mNeonGreen P2A puro

   Plasmid

   #122183

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9 (NEWENTRY )
   mNeonGreen
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

    Plasmid

    #128755

    Purpose

    Expresses CYRANO gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    CYRANO sgRNA (OIP5-AS1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p194-RV-CAG-dio-mScarlet-T2A-Brn2

    Plasmid

    #204741

    Purpose

    Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

    Depositor

    Lynette Lim

    Article

    Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

    Insert

    Brn2
    

    Use

    Retroviral

    Promoter

    CAG

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70

    Plasmid

    #62947

    Purpose

    Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-LexA::QFAD-Hsp70
    

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-AU1

    Plasmid

    #162102

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-StrepTagII-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-StrepTagII-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-FLAG

    Plasmid

    #162104

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-ProtC-FLAG
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-FLAG

    Plasmid

    #162110

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to StrepTagII-ProtC-FLAG
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-AU1

    Plasmid

    #162085

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-ProtC-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEpicVector3G_Puro-T2A-EGFP-VSVg-FLAG-AU1

    Plasmid

    #162088

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-FLAG-AU1

    Plasmid

    #162094

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-FLAG

    Plasmid

    #162081

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-StrepTagII-FLAG
    

    Use

    Lentiviral

    Tags

    VSVg-StrepTagII-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

    Plasmid

    #211115

    Purpose

    For tagging the C-term of Top2A with Venus

    Depositor

    Job Dekker

    Article

    Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

    Inserts

    5' C-term Top2A homology arm (TOP2A Human)
    Venus
    T2A
    Blastocidin Resistance
    5' C-term Top2A homology arm (TOP2A Human)
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    n/a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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