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pAc2_Cas7

Plasmid

#178739

Purpose

Expression of A. chroococcum type I-E #2 cas7

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

A. chroococcum type I-E #2 cas7

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-CAAAG

Plasmid

#170096

Purpose

deGFP reporter plasmid with CAAAG PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-CAATG

Plasmid

#170097

Purpose

deGFP reporter plasmid with CAATG PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-GTAAT

Plasmid

#170098

Purpose

deGFP reporter plasmid with GTAAT PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-GTATT

Plasmid

#170099

Purpose

deGFP reporter plasmid with GTATT PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-ATAAC

Plasmid

#170095

Purpose

deGFP reporter plasmid with ATAAC PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pG-04

Plasmid

#166935

Purpose

Target DC (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

pT181 dual control activator (DC) - Flanked by BsaI restriction sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pG-03

Plasmid

#166934

Purpose

Target AD1.A6 part (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Star sense AD1.S6 - Block G- Flanked by BsaI sites

Use

Synthetic Biology

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTK678

Plasmid

#114707

Purpose

Express NuMA(1-1895)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-1895)-RFP-Nano

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTK652

Plasmid

#114700

Purpose

Express NuMA(1-1700)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-1700)-RFP-Nano

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTK642

Plasmid

#114706

Purpose

Express NuMA(1-213)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-213)-RFP-Nano

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTK641

Plasmid

#114705

Purpose

Express NuMA(1-413)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-413)-RFP-Nano

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTK664

Plasmid

#114704

Purpose

Express NuMA(1-505)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-505)-RFP-Nano

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTK643

Plasmid

#114703

Purpose

Express NuMA(214-705)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(214-705)-RFP-Nano

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTK660

Plasmid

#114702

Purpose

Express NuMA(1-705)4A-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-705)4A-RFP-Nano

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTK631

Plasmid

#114701

Purpose

Express NuMA(1-705)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-705)-RFP-Nano

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_25

Plasmid

#63805

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_14

Plasmid

#63804

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A1NT

Plasmid

#62255

Purpose

expression of A1NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A1NT

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PA5-RFP

Plasmid

#62251

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Expression

Bacterial

Promoter

pA5

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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