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Cas-Acr (insertion 5)

Plasmid

#128116

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (insertion 5)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (N39A)

Plasmid

#128117

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (N39A)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (N39A)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMLS234

Plasmid

#73682

Purpose

SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLY108

Plasmid

#130938

Purpose

Engineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB1

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaBS-sgRNA500

Plasmid

#149615

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaBS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0026-sgRNA500

Plasmid

#149620

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0026

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCJH031_proD_LbCas12a-gRNA-GFP_CAM

Plasmid

#183075

Purpose

Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA targeting GFP

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJZ002

Plasmid

#120231

Purpose

CRISPR-cas9 targeting in E. coli with ampicillin resistance

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Type

Empty backbone

Use

CRISPR

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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BC1364-mouse U6-sgH2B

Plasmid

#164043

Purpose

U6-driven sgRNA targeting H2B

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

H2B targeting sgRNA

Use

CRISPR

Promoter

mouse U6

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA1

Plasmid

#170088

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1050S (UASt-dCasRx)

Plasmid

#132419

Purpose

Express dCasRx under UASt promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Inserts

CasRx
UASt+hsp70

Expression

Insect

Promoter

UASt

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

Plasmid

#159913

Purpose

Mutagenesis of Kcnj6

Depositor

Larry Zweifel

Insert

Kcnj6 (Kcnj6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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S7_SwaI_Zeo_PacI

Plasmid

#128471

Purpose

Zeo Resistance gene

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

Zeocin Resistance gene

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (wtAcrIIA4)

Plasmid

#128115

Purpose

Expresses SpyCas9 fused to wild-type AcrIIA4

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (wtAcrIIA4)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCF204

Plasmid

#121656

Purpose

U6-sgRNA EFS-Cas9-wt-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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S12_EcoRV_PGK_AscI

Plasmid

#128476

Purpose

PGK promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

PGK promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDDIT3.1.0-gDNA

Plasmid

#112396

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DDIT3

Depositor

Kevin White

Insert

DDIT3 (DDIT3 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF503

Plasmid

#135735

Purpose

Donor vector for 3' FLAG tag of human ZNF503

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF503 Homology arms (ZNF503 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBC2102-reci

Plasmid

#117171

Purpose

U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_wtCas9 without NLS

Use

Lentiviral

Promoter

U6 Promoter

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Drosha_4

Plasmid

#79863

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only