We narrowed to 25,574 results for: nol

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MSCV-LMP1-IRES-mCherry

Plasmid

#220351

Purpose

Expresses EBV latent gene LMP1 in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHLuc (Antares-SEPLuc C1A4E+6)

Plasmid

#141089

Purpose

final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

Plasmid

#216696

Purpose

Synchronize trafficking of TNFalpha from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)

Tags

FIREtag, Streptavidin Binding Protein (SBP), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAB1679 CMV-HIVNES-GS-Cas13bt3

Plasmid

#176317

Purpose

CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt3

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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L2092-GPA-FullBRET-Retrovirus-TD142

Plasmid

#222877

Purpose

Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-NanoLuc-CyOFP1

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAM4891 (pCV0003)

Plasmid

#120080

Purpose

Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2

Use

Other

Mutation

mobA Y25F

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMK312 (TOP2A CRISPR)

Plasmid

#140654

Purpose

TOP2A tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBX_RPL7a_smRuby2_HA

Plasmid

#236087

Purpose

Constitutively encodes RPL7a-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting the 60S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPL7a-smRuby2-HA (RPL7A Human, Synthetic)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pF420

Plasmid

#123659

Purpose

Produces cofactor F420 in E. coli

Depositor

Colin Jackson

Article

Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

Inserts

CofD
FbiB
FbiD
FbiC

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_R0040

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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011LP-pAAV-TNT4-EmGFP-pA

Plasmid

#120280

Purpose

AAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoter

Depositor

Xander Wehrens

Article

Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

Insert

EmGFP

Use

AAV

Tags

3XFlag and HA

Promoter

TNT Cardiac Troponin T promoter

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4max-NRRH

Plasmid

#136918

Purpose

Expresses BE4max-NRRH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRRH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSL3927 (pDonor(plasmid)_Pse)

Plasmid

#200905

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (pTarget NGS)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO(ChRger2-TS-YFP)

Plasmid

#127239

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger2-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4max-NRTH

Plasmid

#136919

Purpose

Expresses BE4max-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor

Plasmid

#184951

Purpose

Donor for both PiggyBac and Cp36 Recombinase

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

PuroR, P2A, mCherry

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CABE-T3.1

Plasmid

#193267

Purpose

Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T3.1

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_Chromo_TAF3_Phd

Plasmid

#179400

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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SMC2 CRISPR

Plasmid

#140649

Purpose

SMC2 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

SMC2 (SMC2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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L2125-FRB-GPA-NLuc-CyOFP1

Plasmid

#222885

Purpose

Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc-10GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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