We narrowed to 9,775 results for: pho

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CA-0059 CMVTO-SumIL2-rens-5GS-IL2Rb

Plasmid

#231224

Purpose

CageIL-2 (C-term 25 AA)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

SumIL2-rens-5GS-IL2Rb (IL2 Human)

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-Cdc42-Q61L

Plasmid

#214288

Purpose

Express constitutively active Cdc42 fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Constitutively active Cdc42 (CDC42 Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Cdc42-Q61L

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

Plasmid

#175259

Purpose

Dual plasmid labeling membrane target protein of different neuronal population with different fluorophore

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

Lox-rox-STOP-rox-lyn-mScarlet-pA-lox

Use

Cre/Lox; Dre/rox

Tags

mScarlet

Expression

Mammalian

Mutation

None (wt)

Promoter

pCAG

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159854

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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PA-mCherry1-Vimentin-7

Plasmid

#57202

Purpose

Localization: Intermediate Filaments, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

Insert

Vimentin (VIM Human)

Tags

PA-mCherry1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-His10-SHP1_PTPase (243-541aa)

Plasmid

#180812

Purpose

Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SHP1_PTPase (243-541aa) (PTPN6 Human)

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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Sema4g-Fc-His

Plasmid

#72160

Purpose

Expresses the extracellular region of the Sema4G protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4g (Sema4g Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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Mito-mEosEM-E

Plasmid

#226335

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; mitochondria-targeted mEosEM-E for mammalian expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Insert

COX8A (COX8A Human)

Tags

mEosEM-E

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Sema3g.a(L)-Fc-His

Plasmid

#72151

Purpose

Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.a (Sema3g Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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Dendra2-Vinculin-N-21

Plasmid

#57749

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

NM_003373.3 (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Sema6c-Fc-His

Plasmid

#72167

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H2B-μDpTT

Plasmid

#182328

Purpose

Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

H2B-μDronpa-TagRFP-T (H2BC3 Human)

Tags

Full-length human histone H2B

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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Plxna4-AP-His

Plasmid

#71999

Purpose

Expresses the extracellular region of the PlexinA4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna4 (Plxna4 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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paGFP-HRas G12V

Plasmid

#18694

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

H-Ras G12V (HRAS Human)

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation in paGFP G12V mutation in HRas

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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Neo1.a-AP-His

Plasmid

#71963

Purpose

Expresses the extracellular region of the Neogenin 1, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.a (Neo1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pmEos2-C1-centrin-1

Plasmid

#72588

Purpose

mEos2 photoconvertible fluorescent protein fused to the N-terminus of centrin-1

Depositor

Michel Bornens

Article

Sillibourne et al Cytoskeleton (Hoboken). 2011 Nov;68(11):

Insert

centrin 1 (CETN1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159858

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Sema3e(L)-Fc-His

Plasmid

#72145

Purpose

Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3e (Sema3e Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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MK14_HUMAN_D0

Plasmid

#79680

Purpose

This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK14

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2B_HUMAN_D0

Plasmid

#79718

Purpose

This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2B

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only