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pCLX-UBI-mCherry

Plasmid

#114275

Purpose

Ubiquitous expression of the mCherry living color

Depositor

Patrick Salmon

Article

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

mCherry

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107324

Purpose

Expresses SpCas9 fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to NmCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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mGarnet2-RITA

Plasmid

#104314

Purpose

codes for a fusion of fluorescent protein mGarnet2 and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells

Depositor

Ulrich Nienhaus

Article

Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

Insert

mGarnet2-RITA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pPI278

Plasmid

#113227

Purpose

extrachromosomal expression vector, expression of H2B-GFP

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

H2B

Use

Dictyostelium discoideum expression vector, extra…

Tags

GFP

Promoter

act15

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1488

Plasmid

#108995

Purpose

Cre expression vector

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Cre recombinase

Use

Cre/Lox; Cre expression vector

Promoter

act15

Available Since

July 23, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1038

Plasmid

#108988

Purpose

Inducible expression vector (dox-on)

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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POM42-mTFP1

Plasmid

#69852

Purpose

N-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1 minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pUDE722

Plasmid

#103022

Purpose

expression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crHCAN1-4.S (CAN1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHES873

Plasmid

#87945

Purpose

Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast)

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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sg2.02 MUC4.1

Plasmid

#101152

Purpose

gRNA with two MCP binding sites

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Promoter

mU6

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCAS_gpyrG1

Plasmid

#90276

Purpose

(also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

gRNA (pyrg1)

Use

A. niger

Expression

Bacterial

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBN280

Plasmid

#86731

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Ptph-1::FLP D5

Expression

Worm

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBN266

Plasmid

#86732

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pdpy-7::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA TdL

Plasmid

#80938

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pIM700

Plasmid

#74639

Purpose

alias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

none

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMars1CyeGFP

Plasmid

#75222

Purpose

Mammalian intracellular expression of fluorogen activating protein Mars1Cy-eGFP fusion

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pTnos (GB0037)

Plasmid

#68188

Purpose

Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Nos terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pHsp70B (GB0155)

Plasmid

#68172

Purpose

Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp70B promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pPnos (GB0072)

Plasmid

#68166

Purpose

Provides the Agrobacterium tumefaciens Nopaline synthase promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Nos promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pPML1 (GB0045)

Plasmid

#68164

Purpose

Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtML1 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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