We narrowed to 22,640 results for: his

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pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10

Plasmid

#162388

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8f

Tags

6xHis

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-iSeroSnFR

Plasmid

#128483

Purpose

Fluorescent reporter for serotonin (bacterial expression)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

His, T7, and cpsfGFP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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bNS2B47NS3

Plasmid

#155320

Purpose

co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length

Depositor

Dahai Luo

Article

Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

Insert

Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor

Tags

His tag

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMSP1E2D1

Plasmid

#161606

Purpose

Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E2D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Human pIgR(SC)

Plasmid

#158216

Purpose

Express the ectdomain (SC) of human pIgR

Depositor

Junyu Xiao

Article

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

PIGR (PIGR Human)

Tags

8×his

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pIgnaviCas9

Plasmid

#127595

Purpose

Expresses IgnaviCas9 in E. coli

Depositor

Stephen Quake

Article

Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

Insert

IgnaviCas9

Tags

6xHis Tag and Maltose binding protein

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oPent

Plasmid

#124664

Purpose

Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oPent

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-GFP26

Plasmid

#106406

Purpose

P-RAN-GFP26 (RANbody GFP26::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-GFP26

Tags

HA and His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Aurein1.2_(+36GFP)-Cre

Plasmid

#71748

Purpose

Aurein1.2+36GFP-Cre fusion expression in E. Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_Aurein1.2+36GFP-Cre

Tags

6XHis

Expression

Bacterial

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pAraH6HATT

Plasmid

#63901

Purpose

For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.

Depositor

Carlos Barbas

Article

Barbas et al Methods, A companion to Methods in Enzym

Insert

TT Fab

Tags

6xHis and HA tag

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 WAVE 2

Plasmid

#67098

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

WAVE 2 (WASF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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FcD265A-IL2

Plasmid

#65419

Purpose

Mammalian expression plasmid for murine serum-persistent IL-2

Depositor

Dane Wittrup

Article

Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

Insert

FcD265A-IL2 (Il2 Mouse)

Tags

His

Expression

Mammalian

Mutation

D265A

Promoter

Modified CMV Promoter

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
BglB in pET29b(+)

Plasmid

#55773

Purpose

Expresses beta-glucosidase

Depositor

Justin Siegel

Article

Carlin et al PLoS One. 2016 Jan 27;11(1):e0147596. do

Insert

Beta-glucosidase B (bglB Synthetic)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only

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pQTEV-ANP32A

Plasmid

#31563

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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BRD2

Plasmid

#39074

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BRD2 (BRD2 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
BRD3

Plasmid

#38940

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BRD3 (BRD3 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pCMV5-rat ERK2-WT

Plasmid

#40812

Depositor

Natalie Ahn

Article

Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

Insert

MAPK1 (Mapk1 Rat)

Tags

His6

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pEMB52/Dvl1_1_670

Plasmid

#40542

Depositor

Michael J Fox Foundation MJFF

Insert

DVL1 (DVL1 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pET22b-CPDSacI

Plasmid

#38255

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-Rx3(A7)

Plasmid

#35525

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

His6-Rx3(A7)

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

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