We narrowed to 2,403 results for: T2A

1. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

   Plasmid

   #102888

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-p53 gRNA-Cas9-T2A-mCherry

   Plasmid

   #164256

   Purpose

   Transcription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sg p53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

   Plasmid

   #135014

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Mutation

   126aa domain from HSV-1 UL12 fused to the C-termi…

   Promoter

   CBh

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. mT2A-sfGFP-linker-iCre-ERT2-FNF

   Plasmid

   #182341

   Purpose

   Recombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNF

   Depositor

   Mario Capecchi

   Insert

   T2A-sfGFP-linker-iCre-ERT2-FNF
   

   Use

   Mouse Targeting; Flp / frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LV anti-FITC-LaG16-Y186A-T2A-BFP

   Plasmid

   #194932

   Purpose

   LV construct encoding Y186A mutant sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

   Plasmid

   #135013

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Mutation

   126aa domain from HSV-1 UL12 fused to the N-termi…

   Promoter

   CBh

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLNCX2-NGN2-IRES-GFP-T2A-Sox11

   Plasmid

   #90213

   Purpose

   To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

   Insert

   NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Mouse, Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

   Plasmid

   #31477

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV HA NLS Y2 T2A mTagBFP
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Expression

   Mutation

   F13Y + S111Y on I-AniI = Y2 "reo" (res…

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-ProtC

   Plasmid

   #162099

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-StrepTagII-ProtC
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-ProtC

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid

    #87827

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

    Plasmid

    #60492

    Purpose

    Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm

    Depositor

    Dorus Gadella

    Article

    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

    Inserts

    3xnls-SGFP2(T65G)
    SGFP2-3xNES
    

    Use

    Tags

    SGFP2 and SGFP2(T65G)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2

    Plasmid

    #60626

    Purpose

    Expresses VP16 mouse MyoD and dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    VP16 mouse MyoD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. LV anti-FITC-LaG16-R103A-T2A-BFP

    Plasmid

    #194935

    Purpose

    LV construct encoding R103A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

    Plasmid

    #165030

    Purpose

    Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2ADW-lox-mCherry-hyBRET-ERK-NLS

    Plasmid

    #170290

    Purpose

    For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    lox-mCherry-lox-hyBRET-ERK-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTY153 EGFP-T2A-ORF6 SARS-CoV-2

    Plasmid

    #204975

    Purpose

    Co-express EGFP and SARS-CoV-2 ORF6

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

    Plasmid

    #175521

    Purpose

    Nuclear expression of HaloTag10 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTY152 EGFP-T2A-ORF6 SARS-CoV-1

    Plasmid

    #204974

    Purpose

    Co-express EGFP and SARS-CoV-1 ORF6

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-CAG-FLEX-HA-DLX2-T2A-mCherry

    Plasmid

    #178585

    Purpose

    Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    DLX2 (DLX2 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBbi-moxGFP-P2A-T2A-cMyc-Puro

    Plasmid

    #176892

    Purpose

    Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Article

    Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

    Insert

    moxGFP-P2A-T2A-c-Myc
    

    Use

    Transposon

    Tags

    Myc and moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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