We narrowed to 1,429 results for: puc57

1. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

   Plasmid

   #46168

   Purpose

   Codon optimized Cas9_SV40 NLS with intron

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   codon optimized Cas9_SV40 NLS with intron
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   intron inserted at position 3113-3163, and SV40 N…

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP

   Plasmid

   #230075

   Purpose

   Partial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

   Depositor

   Sumeet Pal Singh

   Article

   Hovhannisyan et al bioRxiv 2025.05.23.655316

   Insert

   lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   fabp10a

   Available Since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSL3936 (pDonor(AAVS1)_Pse)

   Plasmid

   #200903

   Purpose

   Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   Mini Tn7016 (AAVS1 NGS)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDGE334

   Plasmid

   #153243

   Purpose

   M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTS2355_Tier3(PB)-iRFP_PuroR

   Plasmid

   #169654

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven iRFP670 and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRPBSA

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDGE332

   Plasmid

   #153241

   Purpose

   M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDGE333

   Plasmid

   #153242

   Purpose

   M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDGE335

   Plasmid

   #153244

   Purpose

   M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-Cj-sgRNA

   Plasmid

   #89753

   Purpose

   U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   Cj-SgRNA
   

   Use

   Sgrna expression under u6 promoter

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   May 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGE337

    Plasmid

    #153246

    Purpose

    M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

    Plasmid

    #205447

    Purpose

    human AAVS1-targeting plasmid for expression of mouse CR1

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDGE497

    Plasmid

    #153249

    Purpose

    M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDGE496

    Plasmid

    #153248

    Purpose

    M6 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGE495

    Plasmid

    #153247

    Purpose

    M5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDGE336

    Plasmid

    #153245

    Purpose

    M4 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGE341

    Plasmid

    #153250

    Purpose

    M7 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGE342

    Plasmid

    #153251

    Purpose

    M8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC-fFuCas9-HTBNLS-hph

    Plasmid

    #121092

    Purpose

    Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

    Depositor

    Xiao-Jun Ji

    Article

    Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

    Insert

    Cas9 and hygromycin resist gene
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    gpdA;trpC

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRExT2A-NT-PmNG

    Plasmid

    #232200

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mNeongreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TC1154

    Plasmid

    #164876

    Purpose

    Split GFP reporter plasmid expressing GFP (b11beta

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    Split GFP(beta11)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge