215,910 results

1. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

   Plasmid

   #32702

   Purpose

   Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Insert

   eGFP
   

   Use

   Cre/Lox and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

   Plasmid

   #32702

   Purpose

   Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Insert

   eGFP
   

   Use

   Cre/Lox and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCZGY546

   Plasmid

   #90466

   Purpose

   Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Pmec-4-mcherry
   

   Tags

   mcherry

   Expression

   Worm

   Promoter

   Pmec-4

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF2545

   Plasmid

   #144038

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CEBPB (CEBPB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

   Plasmid

   #115519

   Purpose

   To construct multiple sgRNA expression vector for CRISPR

   Depositor

   Xingxu Huang

   Insert

   ccdB-sgRNA scaffold
   

   Use

   CRISPR

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-Cas9-GFP

   Plasmid

   #86145

   Purpose

   Lentiviral vector expressing Cas9-GFP

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   Cas9-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBABE-MCS(extended)-Puro (empty vector)

   Plasmid

   #172603

   Purpose

   Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   The multiple cloning site (MCS) has been edited/e…

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAC-BETAipi

   Plasmid

   #53277

   Purpose

   Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

   Insert

   idi
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   endogenous promoter

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAC-BETAipi

   Plasmid

   #53277

   Purpose

   Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

   Insert

   idi
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   endogenous promoter

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Myc-E-Syt2

    Plasmid

    #66834

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt2 (ESYT2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

11. Myc-E-Syt2

    Plasmid

    #66834

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt2 (ESYT2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pINDUCER20_3xFlag

    Plasmid

    #190028

    Purpose

    Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal 3xFlag

    Depositor

    Andrew Tutt

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZac2.1 hSynapsin1 NAPA-N SV40

    Plasmid

    #92282

    Purpose

    FRET probe; extracellular synapse targeted mCherry

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Neuronal
    

    Use

    AAV

    Promoter

    hSynapsin1

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-CMV-MCS-Puro-EGFP

    Plasmid

    #219795

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-Q23

    Plasmid

    #40261

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q23 (HTT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-Q23

    Plasmid

    #40261

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q23 (HTT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCytochrome C-GFP

    Plasmid

    #41182

    Depositor

    Douglas Green

    Article

    Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

    Insert

    Cytochrome C
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCytochrome C-GFP

    Plasmid

    #41182

    Depositor

    Douglas Green

    Article

    Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

    Insert

    Cytochrome C
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMKV057

    Plasmid

    #133312

    Purpose

    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPT

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc+. WUS2, IPT
    

    Expression

    Plant

    Promoter

    AtUbi10, Nos, 35S

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX552

    Plasmid

    #60958

    Purpose

    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Inserts

    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Expression

    Mammalian

    Promoter

    Synapsin and U6

    Available Since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge