We narrowed to 90,284 results for: Mal;

1. MSP2372

   Plasmid

   #73196

   Purpose

   Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, and Q926A in SpCas9

   Promoter

   CMV

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3flag-RanGAPdeltaN

   Plasmid

   #25104

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   RanGAP1 deleted N-term domain (RANGAP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-203.

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pAVA-LGF2-Gal11p

   Plasmid

   #127481

   Purpose

   Positive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interaction

   Depositor

   Joel Malek

   Article

   Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

   Insert

   LGF2-Gal11p (GAL11 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAd GFP HDAC5 S259A/S498A

   Plasmid

   #32218

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Use

   Adenoviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S259A/S498A

   Available Since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

5. DCT.VV.Orf37

   Plasmid

   #12725

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf37
   

   Expression

   Mammalian

   Available Since

   Dec. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

6. DCT.VV.Orf48

   Plasmid

   #12736

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf48
   

   Expression

   Mammalian

   Available Since

   Dec. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pMuLE ENTR CMV CreERT2 R4-R3

   Plasmid

   #62173

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   CreERT2
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

   Plasmid

   #188993

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   D201G, A258T, K327R, G328S, G381V, G429D, T450A

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. G1333 DddAtox-C–SaKKH-Cas9(D10A)

   Plasmid

   #157838

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Expression

   Mammalian

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Dynamin I-K44A-non-tagged

    Plasmid

    #32695

    Depositor

    Robert Lefkowitz

    Article

    Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

    Insert

    Dynamin I (Dnm1 Rat)
    

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    CMV

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. FKBP12-CB2x2-mTagBFP2-HAx2

    Plasmid

    #207146

    Purpose

    Tandem calcium-buffer with rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Calbindin-2 (CALB2 Human)
    

    Tags

    FKBP12, HA, and mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_SNORD27h

    Plasmid

    #73066

    Purpose

    expression clone for human SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (SNORD27 Human)
    

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    expression cassette for SNORD27

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-SFFV-RAB10-WPRE-UbC-Emerald

    Plasmid

    #203804

    Purpose

    Lentiviral vector plasmid expressing human RAB10 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    RAB10 (RAB10 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-HypaCas9-TUBA1B_sgRNA

    Plasmid

    #183889

    Purpose

    pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSH231-EF1-BLST-Cas9-VPR

    Plasmid

    #115147

    Purpose

    Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    BlastR-P2A-Cas9-VPR
    

    Use

    CRISPR

    Tags

    SV40NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. C1-F-tractin(delta1-6)-mCitrine

    Plasmid

    #155219

    Purpose

    F-actin bidning mutant of F-tractin

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)
    

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Deletion of first 6 AA from F-tractin (9-14 from …

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD765 FDFT1 3'UTR

    Plasmid

    #105765

    Purpose

    Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

    Depositor

    Joseph Ziegelbauer

    Article

    Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

    Insert

    FDFT1 3'UTR (FDFT1 Human)
    

    Use

    Luciferase

    Mutation

    None

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAVA-Gal11p-LGF2

    Plasmid

    #127480

    Purpose

    Positive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    Gal11p-LGF2 (GAL11 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHDR-eGFP (pXK-1647)

    Plasmid

    #208824

    Purpose

    The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR

    Depositor

    Kun Xu

    Article

    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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