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RF10

Bacterial strain

#62076

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. lysA genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Publication

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGLTR-X-GFP

Plasmid

#58245

Purpose

GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP

Depositor

Stephan Geley

Publication

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Inserts

attR1-Cm(R)-CcdB-attR2
TetR-NLS-T2A-GFP

Use

Lentiviral and RNAi; Gateway destionation vector

Tags

GFP, NLS, T2A, and TetR

Expression

Mammalian

Promoter

SFFV and no

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmcherry-LCa

Plasmid

#53972

Purpose

Expresses the light chain of clathrin

Depositor

Tom Kirchhausen

Publication

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

clathrin light chain

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
Myc-Rab35 Q67L

Plasmid

#47434

Depositor

Peter McPherson

Publication

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 Q67L (RAB35 Human)

Tags

Myc

Expression

Mammalian

Mutation

Q67L active

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mKate1.31-Lifeact-7

Plasmid

#54668

Purpose

Localization: Actin, Excitation: 588, Emission: 635

Depositor

Michael Davidson

Publication

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

LifeAct

Tags

mKate1.31 (aka mKate2)

Expression

Mammalian

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pWZLblasti-HA-ERK2 GOF

Plasmid

#53174

Purpose

Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Publication

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Mustard Weed, Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S/D321N

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pWZLblasti-HA-ERK5

Plasmid

#53175

Purpose

Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Publication

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK5 (MAPK7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
SB51

Plasmid

#48184

Purpose

Express full length HA tagged p35sr in mammalian cells

Depositor

Shoumo Bhattacharya

Publication

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFA6a-12Pk-hphMX6

Plasmid

#49177

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

12Pk

Expression

Yeast

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pRBBm34

Plasmid

#48114

Purpose

Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megaterium

Depositor

Dieter Jahn

Publication

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAC5-dual-dCas9VP48-sgTetO

Plasmid

#48237

Purpose

Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48 and sgTetO

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-5X2-RalBP1

Plasmid

#37245

Depositor

Christopher Counter, David Kashatus

Publication

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

RalBP1 (RALBP1 Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA4-FLAG-Jhdm2a-H1122A

Plasmid

#38140

Depositor

Toshinobu Nakamura, Toru Nakano

Publication

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Insert

Jhdm2a-H1122A (Kdm3a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115; H1122A

Promoter

CMV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-DUET-GST-eIF4G1-His6, eIF4E

Plasmid

#37232

Depositor

Jon Lorsch

Publication

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Inserts

TIF4631 (TIF4631 Budding Yeast)
CDC33 (CDC33 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pGEX 4T1 ERbeta

Plasmid

#35563

Depositor

Harish Srinivas

Publication

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Tags

GST

Expression

Bacterial

Available since

March 26, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP_Rab8a

Plasmid

#31803

Depositor

Daniel Gerlich

Publication

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

Rab8a (RAB8A Human)

Expression

Mammalian

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pLenti6/V5-DEST-NCAPH

Plasmid

#31214

Depositor

Lynda Chin

Publication

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NCAPH (NCAPH Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-Bax1-2/L-6

Plasmid

#30533

Depositor

Richard Youle

Publication

Edlich et al Cell. 2011 Apr 1. 145(1):104-16.

Insert

BCL2-associated X protein (BAX Human)

Tags

eGFP

Expression

Mammalian

Mutation

F30C E44C C62S L63C C126S P130C

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGEX-GST-AGO3

Plasmid

#24318

Depositor

Carl Novina

Publication

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

AGO3 (AGO3 Human)

Tags

GST

Expression

Bacterial

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
hOtub1

Plasmid

#26959

Depositor

Cynthia Wolberger

Publication

Wang et al J Mol Biol. 2009 Mar 6. 386(4):1011-23.

Insert

Otub1 (OTUB1 Human)

Tags

His6 (TEV cleavable)

Expression

Bacterial

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles