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We narrowed to 16,215 results for: grna

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Showing: 9241 - 9260 of 16215 results

  2. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139097
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139097-plasmid-map-sequence-id-273896

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  3. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139095
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139095-plasmid-map-sequence-id-273892

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  5. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139088
    Purpose
    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T, R324L,S409I, E480K, E543D, M694I, E1219V
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139088-plasmid-map-sequence-id-273884

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  8. PB-EEA-g1-PGK-Puro

    Plasmid
    #102908
    Purpose
    PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EEA-guide1-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102908-plasmid-map-sequence-id-198395

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  9. GG-EBNA-EEA-guide1-PGK-Puro

    Plasmid
    #102904
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EEA-guide1-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102904-plasmid-map-sequence-id-194742

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  12. pLenti-ePegRNA-acceptor-mCherry

    Plasmid
    #260209
    Purpose
    Lentiviral backbone to clone pegRNA with puromycin resistance and mCherry expression, encodes for mRFP for cloning selection
    Depositor
    Leopold Parts
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    mCherry
    Available since
    Sept. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    260209-plasmid-map-sequence-id-521742

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  20. pTBL3440 MTK_PBdest lacO-hU6-BtoApegRNA-LacI-mCherry

    Plasmid
    #254862
    Purpose
    peCHYRON destination vector expressing IPTG-inducible B-to-A pegRNA, LacI, and mCherry. Can be integrated into the genome of mammalian cells with PiggyBac transposase.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d
    Inserts
    lacO_hU6_promoter-peCHYRON_BtoA_pegRNA
    pCAG-LacI-rb_glob_polyA
    pEF1alpha-mCherry-BGH_polyA
    Use
    Piggybac integration
    Expression
    Mammalian
    Available since
    June 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    254862-plasmid-map-sequence-id-512990

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Showing: 9241 - 9260 of 16215 results