Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,788 results for: pho

Clear keywords
Showing: 9241 - 9260 of 9788 results

  3. pEVOL-ABK

    Plasmid
    #126035
    Purpose
    Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:
    Inserts
    tRNA synthetase 1
    tRNA synthetase 2
    tRNA for TAG codon
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    araBAD promoter, glnS promoter, and proK promoter
    Available Since
    Sept. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    126035-plasmid-map-sequence-id-244699

    Enlarge

  8. His-DNPH1

    Plasmid
    #203224
    Purpose
    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    DNPH1 (DNPH1 Human)
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203224-plasmid-map-sequence-id-400075

    Enlarge

  12. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid
    #195741
    Purpose
    Base editing for making a pre-stop codon in mmoX using phenol inducible system
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Po (phoenol-inducible promoter)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195741-plasmid-map-sequence-id-386423

    Enlarge

  13. SiriusGFP

    Plasmid
    #123201
    Purpose
    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.
    Depositor
    Joseph Santos-Sacchi
    Article
    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76
    Insert
    SiriusGFP
    Use
    Mouse Targeting
    Tags
    N/A
    Expression
    Bacterial and Mammalian
    Mutation
    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…
    Promoter
    CMV
    Available Since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    123201-plasmid-map-sequence-id-239192

    Enlarge

  14. pGLACTE-PseudoHT51

    Plasmid
    #99257
    Purpose
    Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresis
    Depositor
    Karen Usdin
    Article
    Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi
    Insert
    FMR1 (FMR1 Human, Synthetic)
    Mutation
    Contains synthetic, non-human, primer sequence do…
    Available Since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    99257-plasmid-map-sequence-id-181641

    Enlarge

  18. L2096-GPA-BRET-Biosensor-Retrovirus-TD146

    Plasmid
    #222878
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    MSCV LTR
    Available Since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222878-plasmid-map-sequence-id-446610

    Enlarge

Showing: 9241 - 9260 of 9788 results