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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,831 results for: sequence

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Showing: 9301 - 9320 of 13831 results

  1. pHSN401

    Plasmid
    #50588
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    maize codon optimized
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50588-plasmid-map-sequence-id-75660

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  4. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

    Plasmid
    #137830
    Purpose
    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor
    Depositor
    Joshua Leonard
    Article
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Insert
    FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Leucine 71 to Lysine in CTEVp fragment
    Promoter
    CMV
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137830-plasmid-map-sequence-id-311956

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  8. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

    Plasmid
    #207634
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
    Expression
    Mammalian
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207634-plasmid-map-sequence-id-413133

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  9. pBUN411

    Plasmid
    #50581
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    maize codon optimized
    Promoter
    OsU3p and Ubi1p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50581-plasmid-map-sequence-id-311045

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  11. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid
    #117274
    Purpose
    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i
    Depositor
    David Stephens
    Article
    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180
    Inserts
    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    Tags
    mScarlet-i
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV and IRES
    Available Since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    117274-plasmid-map-sequence-id-233028

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  14. pTP261

    Plasmid
    #104166
    Purpose
    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling
    Depositor
    Dirk Görlich
    Article
    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554
    Insert
    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    Tags
    14x Histidine tag and NEDD8 from Brachypodium dis…
    Expression
    Bacterial
    Mutation
    1x Cysteine located at bps 493-495 in full sequen…
    Available Since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    104166-plasmid-map-sequence-id-200075

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  20. MuHKII-pGFPN3

    Plasmid
    #21922
    Depositor
    Hossein Ardehali
    Article
    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.
    Insert
    Mutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This is the full length human HKII cDNA sequence …
    Available Since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
Showing: 9301 - 9320 of 13831 results