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Plasmid#127565PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.DepositorInsertp65
UseCRISPRExpressionInsectAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
Plasmid#125225PurposeExpresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cellsDepositorInsertgLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
UseAAVTagsHAExpressionMammalianPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX208-gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
Plasmid#125233PurposeExpresses "sender" trans-cellular NanoLuc in mammalian cellsDepositorInsertgLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
UseLentiviralTagsHAExpressionMammalianPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
JL202: pMVP/mCherry-DEST
Plasmid#121853PurposepMVP destination vector, empty expression vector backbone w/ mCherry selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorExpressionMammalianAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125231PurposeFor generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirusDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseLentiviralTagsHAExpressionMammalianPromoterCMVAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-eGFP-B-actin
Plasmid#117812PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyTagsNLSAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-TagRFP-B-actin
Plasmid#117806PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagRFP
UseSynthetic BiologyTagsNLSAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN mEGFP-OPTN(F178A)
Plasmid#119681PurposeFor mammalian expression of LIR mutant OPTNDepositorAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaCTD_P2A_Hygro_Barcode
Plasmid#120511PurposeBarcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaCTD (MYC Human)
UseLentiviralMutationDeletion of carboxy-terminal domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaNTD_P2A_Hygro_Barcode
Plasmid#120510PurposeBarcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaNTD (MYC Human)
UseLentiviralMutationDeletion of amino-terminal domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaMBI_P2A_Hygro_Barcode
Plasmid#120504PurposeBarcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-181a-5p
Plasmid#103276PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-181a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-181a-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-101-3p
Plasmid#103165PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-101-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-101-3p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)
Plasmid#115140PurposeCMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLSDepositorInserthuman codon optimized AcrVA3 anti-CRISPR protein
TagsNLS(SV40)ExpressionMammalianAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)
Plasmid#115138PurposeCMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLSDepositorInserthuman codon optimized AcrVA2 anti-CRISPR protein
TagsNLS(SV40)ExpressionMammalianAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pROS17
Plasmid#107931PurposeTRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pROS16
Plasmid#107930PurposeHIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pROS11
Plasmid#107925PurposeamdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-HIS-TagGFP2-TEV-GGG-ELP(40nm)-Cys
Plasmid#90474PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal TagGFP2 and TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertTagGFP2-TEV-GGG-ELP(40nm)-Cys
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-RAP2A-GFP
Plasmid#86124PurposeRetroviral vector expressing RAP2A-GFP-IRES-BsdDepositorAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only