-
Plasmid#169979PurposepDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F370G) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPAM, F370GPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND S297A
Plasmid#169966PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297APromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM F405G
Plasmid#169981PurposepDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F405G) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPAM, F405GPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM E274R
Plasmid#169977PurposepDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPAM, E274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR 221-PHL2 (STOP)
Plasmid#118683PurposeGateway entry vectorDepositorInsertPHL2 (AT3G24120 Mustard Weed)
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR 221-XERICO (STOP)
Plasmid#118685PurposeGateway entry vectorDepositorInsertXERICO (XERICO Mustard Weed)
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR 221-BPC4 (STOP)
Plasmid#118680PurposeGateway entry vectorDepositorInsertBPC4 (BPC4 Mustard Weed)
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 delta787-843
Plasmid#166821PurposeGateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutation_787-843PromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Ub-NLRP1 S1213 P1214R
Plasmid#166830PurposepDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsUbiquitinExpressionMutationP1214RPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TAF15 g1172a No Stop
Plasmid#84898PurposeDONOR vector for Gateway cloning of TAF15 g1172a No StopDepositorInsertTAF15 (TAF15 Human)
UseGateway donorTagsExpressionMutationg1172a No StopPromoternoneAvailable sinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 g1750a No Stop
Plasmid#84894PurposeDONOR vector for Gateway cloning of EWSR1 g1750a No StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationg1750a No StopPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 g1750a Stop
Plasmid#84893PurposeDONOR vector for Gateway cloning of EWSR1 g1750a StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationg1750aPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 c1655t No Stop
Plasmid#84892PurposeDONOR vector for Gateway cloning of EWSR1 c1655t No StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationc1655t No StopPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 c1655t Stop
Plasmid#84891PurposeDONOR vector for Gateway cloning of EWSR1 c1655t StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationc1655tPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 g1532c No Stop
Plasmid#84890PurposeDONOR vector for Gateway cloning of EWSR1 g1532c No StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationg1532c No StopPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10-G1238A-C1240T
Plasmid#24739DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationG replaced with A at position 1238 and C replaced…PromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pT7NT*-Bri2 113-231 R221E
Plasmid#138134PurposeExpresseds human Bri2 BRICHOS R221E mutant in E. coliDepositorInserthuman Bri2 BRICHOS R221E (ITM2B Human)
UseTagsNT* tag derived from spider silk proteinsExpressionBacterialMutationPromoterT7Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 743 (pDONR221-EYFP-hKRAS4B-WT)
Plasmid#156402PurposeGateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codonDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseGateway cloningTagsEYFPExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ108 - pENTR221 - [cbr-unc-119(+)]
Plasmid#200367Purposecbr-unc-119 rescue gene for use as co-injection marker.DepositorInsert[cbr-unc-119(+)]
UseTagsExpressionWormMutationPromoterAvailable sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3
Plasmid#87906PurposesgRNA targeting GFPDepositorInsertsgGFP1, sgGFP2, sgGFP3
UseTagsExpressionMammalianMutationPromoterH1, U6, 7SKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
221-RPB1-AID-eGFP-2A-blasticidin
Plasmid#195107PurposeTargeting vector to introduce AID-eGFP tags in C-terminal of the human RPB1 gene. Blasticidin selection (2A-fusion). Auxin-inducible degron system.DepositorInsertAID-eGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9
Plasmid#87917PurposeGateway cloningDepositorInsertsgHTT1, sgGFP2, shCas9
UseTagsExpressionMammalianMutationPromoterH1, U6, 7SKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
VV221: sdChR(C138S)-TS-eGFP-ER in fck
Plasmid#58513PurposeExpresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoterDepositorInsertsdChR(C138S)-TS-eGFP-ER
UseLentiviralTagseGFPExpressionMammalianMutationchanged Cysteine 138 to SerinePromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)
Plasmid#156405PurposeGateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codonDepositorInserthuman wild type KRAS4A-WT (KRAS Human)
UseGateway cloningTagsmScarlet-IExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K462-463-651R
Plasmid#84564PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK462-463-651RPromoternoneAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K43-117-118R
Plasmid#84565PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK43-117-118RPromoternoneAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
SOX D221A/E244A/K246A pACEmam1
Plasmid#192050PurposeExpresses a 3X catalytic mutant of SOX (ORF37)DepositorInsertSOX (3X catalytic mutant)
UseUnspecifiedTagsExpressionMutation3X catalytic mutant D221A/E244A/K246APromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE SOX (ORF37) D221N E244Q
Plasmid#192053PurposeExpresses a 2X catalytic mutant of (ORF37) D221N E244QDepositorInsertSOX (D221N E244Q)
UseUnspecifiedTagsExpressionMutation2X catalytic mutant D221N E244QPromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-WPRE-bGH-pA (JDW 1221)
Plasmid#224527PurposeA Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRNDepositorInsertWPRE-bGH
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Lifeact- mScarlet-HA (JDW 1247)
Plasmid#224538PurposeA Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)DepositorInsertLifeact-mScarlet-HA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 msNLRP3(ΔPYD, I125M-END)
Plasmid#218641PurposeGateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseGateway entryTagsExpressionMutationdelPYD (I125M-END)PromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K40R-short-UBASH3A-Ntag
Plasmid#195401PurposeHuman K40R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationK40RPromoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K40R-short-UBASH3A-Ctag
Plasmid#195400PurposeHuman K40R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationK40RPromoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-deltaUBA-short-UBASH3A-Ctag
Plasmid#195397PurposeHuman short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationUBASH3A mutant lacking the UBA domainPromoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-W279A-short-UBASH3A-Ctag
Plasmid#195402PurposeHuman W279A short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationW279APromoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K202R-short-UBASH3A-Ctag
Plasmid#195399PurposeHuman K202R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationK202RPromoterAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-deltaUBA-short-UBASH3A-Ntag
Plasmid#195398PurposeHuman short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationUBASH3A mutant lacking the UBA domainPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K202R-long-UBASH3A-Ntag
Plasmid#192093PurposeHuman K202R long-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseTagsExpressionMammalianMutationK202RPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-W279A-short-UBASH3A-Ntag
Plasmid#192096PurposeHuman W279A short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseTagsExpressionMammalianMutationW279APromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K202R-short-UBASH3A-Ntag
Plasmid#192094PurposeHuman K202R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseTagsExpressionMammalianMutationK202RPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-W279A-long-UBASH3A-Ntag
Plasmid#192095PurposeHuman W279A long-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseTagsExpressionMammalianMutationW279APromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBA/UIM/UBM-mut
Plasmid#187153PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationL2976A/P2977A/M1328A/F1330A/A1376G/S1382APromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND F405G + S297A
Plasmid#169974PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, F405GPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND E274R + S297A
Plasmid#169968PurposepDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, E274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND R394E + S297A
Plasmid#169972PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, R394EPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR 221-PHL2 (NON-STOP)
Plasmid#118682PurposeGateway entry vectorDepositorInsertPHL2 (AT3G24120 Mustard Weed)
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR 221-XERICO (NON-STOP)
Plasmid#118684PurposeGateway entry vectorDepositorInsertXERICO (XERICO Mustard Weed)
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR 221-AIP2 (NON-STOP)
Plasmid#118678PurposeGateway entry vectorDepositorInsertAIP2 (AT5G20910 Mustard Weed)
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR 221-FG1 (NON-STOP)
Plasmid#118681PurposeGateway entry vectorDepositorInsertAT3G07200 (AT3G07200 Mustard Weed)
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 FIIND (F1079M-L1373)
Plasmid#166831PurposeGateway pDONR vector containing NLRP1 FIIND (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationPromoterAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only