We narrowed to 8,978 results for: ef1;

1. miniTol2 x EF1a_EKAREN5 + PGK-blast

   Plasmid

   #167818

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-EF1a-BCL2-hygro

   Plasmid

   #222581

   Purpose

   PiggyBac transposon plasmid for constitutive expression of BCL2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   BCL2 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-PGD2-1.1

   Plasmid

   #214764

   Purpose

   AAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoter

   Depositor

   Yulong Li

   Article

   Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

   Insert

   GRAB-PGD2-1.1 sensor
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF-1-alpha promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-DIO-HTB

   Plasmid

   #44187

   Depositor

   Edward Callaway

   Insert

   Histone tagged GFP, T2A-TVA-E2A-B19G
   

   Use

   AAV; Adeno-associated vector

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

5. piggybac-EF1-halo-mTBP

   Plasmid

   #197908

   Purpose

   single-particle tracking for mTBP

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   TBP (Tbp Mouse)
   

   Use

   Tags

   Halotag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98972

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

   Plasmid

   #98973

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 2 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a-TALEN_HD (SPC right)

   Plasmid

   #126695

   Purpose

   right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

   Depositor

   Darrell Kotton

   Article

   Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

   Insert

   human SPC right
   

   Use

   TALEN

   Tags

   NLS, truncated N term and TAL, FokI

   Expression

   Mammalian

   Mutation

   R451P in SPC

   Promoter

   EF1

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a-TALEN_NN (SPC left)

   Plasmid

   #126694

   Purpose

   left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

   Depositor

   Darrell Kotton

   Article

   Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

   Insert

   human SPC left
   

   Use

   TALEN

   Tags

   0.5 repeat NN, TAL, FokI and NLS, truncated N ter…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p1977 (pUC19-PTDH3-PTEF1)

    Plasmid

    #78230

    Purpose

    Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Bidirectional promoters pTDH3-pTEF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pRRLNeo-pEF1a-p53ashL344P-mCerulean

    Plasmid

    #69581

    Purpose

    Expresses p53-L344P mutant tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents oliogomerization…

    Promoter

    EF1alpha

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-EF1a-GFP ArgSen

    Plasmid

    #184695

    Purpose

    Lentiviral transfer plasmid for expression of GFP ArgSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP ArgSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lv EF1a MS2-Fkbp1x 2A Hygro

    Plasmid

    #102806

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp1x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    MS2-Fkbp
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a CHR2 mCherry WPRE

    Plasmid

    #129432

    Purpose

    expression of CHR2 mCherry from EF1a promoter

    Depositor

    Oskar Laur

    Insert

    CHR2 mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1A-CD19.8H.28TM.28.BB.3z

    Plasmid

    #200676

    Purpose

    Modular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

    Plasmid

    #167819

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV Ef1a-mRuby3-WPRE

    Plasmid

    #135427

    Purpose

    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

    Depositor

    Rylan Larsen

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid

    #60600

    Purpose

    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    CRISPR Cas9 D10A
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Changed Asp 10 to Ala, Codon usage optimized for …

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1α1.1-Chronos-tdTomato

    Plasmid

    #122099

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α (1.1 kb short version)

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFIN-EF1a-GFP-WPRE

    Plasmid

    #44352

    Depositor

    Susan Semple-Rowland

    Article

    Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

    Insert

    EF1a-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human elongation factor -1a (pos 2526-3969)

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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