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SIRT7 Flag

Plasmid

#13818

Purpose

Mammalian expression of human SIRT7 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT7 (SIRT7 Human)

Tags

flag

Expression

Mammalian

Mutation

Missing exon 2

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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Halo-Rab6A

Plasmid

#190171

Purpose

Expresses HaloTag-Rab6A in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

HaloTag-Rab6A

Tags

HaloTag

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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EndophilinA1-mRuby2

Plasmid

#171945

Purpose

Expresses Endophilin A1 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Endophilin A1 (SH3GL2 Human)

Tags

mRuby2

Expression

Mammalian

Available Since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRH2521

Plasmid

#84380

Purpose

Expression of sgRNA from a imyc promoter (Pimyc)

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

sgRNA cloning site

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY3196

Plasmid

#172696

Purpose

Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

Prgef-1

Tags

GFP 1-10

Expression

Worm

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3C2G

Plasmid

#171946

Purpose

Expresses PIK3C2G in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

PIK3C2G (PIK3C2G Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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His-TEV-SNAP-PRC1

Plasmid

#167541

Purpose

For expression of His6-TEV-SNAP-PRC1 in bacteria.

Depositor

Tarun Kapoor

Article

Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

Insert

PRC1 (PRC1 Human)

Tags

His6 tag and SNAP tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-hTRIM9

Plasmid

#51032

Purpose

entry vector for cloning human TRIM9

Depositor

Timothy Cox

Article

Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

Insert

TRIM9 (TRIM9 Human)

Use

Entry vector

Promoter

NA

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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Plk1 FRET sensor c-jun substrate

Plasmid

#45203

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Plk1 FRET sensor with c-jun substrate

Tags

CyPET, YPET

Expression

Mammalian

Promoter

T7

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pCZGY405

Plasmid

#172694

Purpose

Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

rab-3 (rab-3 Nematode)

Tags

Punc-25 and mcherry

Expression

Worm

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-eDHFR

Plasmid

#176201

Purpose

Negative control of targeted optogenetic manipulation using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-eDHFR

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEPV-DsRed2-ER-5

Plasmid

#170571

Purpose

Expresses DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

EPV-DsRed2-ER-5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pYC1876

Plasmid

#158722

Purpose

Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

ssrA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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N-term Hec1 Plk1 FRET sensor c-jun substrate

Plasmid

#45204

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted Plk1 FRET sensor

Tags

CyPET, YPET

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pFH54

Plasmid

#128183

Purpose

Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJES102

Plasmid

#135409

Purpose

E. coli 'blaTEM-1 (without signal sequence) that can be used as a reporter

Depositor

Miriam Braunstein

Article

McCann et al Microbiology. 2007 Oct;153(Pt 10):3350-9

Insert

blaTEM-1

Expression

Bacterial

Promoter

Unknown

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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jk089_h12

Plasmid

#112151

Purpose

C. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

C. thermophilum DASH/Dam1 complex

Tags

his tag on Spc34 and strep tag on Dad1

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAcUW31-M10.5/human b2m

Plasmid

#11521

Depositor

Pamela Bjorkman

Article

Olson et al PLoS Biol. 2005 Aug . 3(8):e257.

Insert

M10.5 (H2-M10.4 Mouse)

Tags

His

Expression

Insect

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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L1P63

Plasmid

#195938

Purpose

PhiC31-intein(DnaE-Npu) and Intein(DnaE-Ssp)-PhiC31 : pUBQ10-PI-tUBQ1-p35S-IP-t35S

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10-PI-tUBQ1-p35S-IP-t35S

Expression

Plant

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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L1P5

Plasmid

#195920

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-DST-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3-PhiC31-NLS-DST-tUBQ1

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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