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pCASP-HilA empty vector

Plasmid

#153333

Purpose

Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Type

Empty backbone

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS019E: Entry vector for: ClopHensorN chloride sensor (cytosolic)

Plasmid

#163066

Purpose

Entry vector for: ClopHensorN chloride sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ClopHensorN

Use

Gateway entry vector

Tags

tdTomato

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSiP1

Plasmid

#21952

Depositor

Klaus Rajewsky

Article

Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

Insert

Pten (Pten Mouse)

Expression

Mammalian

Available Since

March 17, 2010

Availability

Academic Institutions and Nonprofits only
2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

Plasmid

#172224

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tpr-Si

Plasmid

#60918

Purpose

Expresses Flag tagged nucleoporin Tpr resistant to siRNA GCACAACAGGATAAGGTTA

Depositor

Vinay Nandicoori

Article

Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

Insert

TPR (TPR Human)

Expression

Mammalian

Mutation

Silent point mutations in siRNA target region; E1…

Promoter

CMV

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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MTK0_043

Plasmid

#123943

Purpose

Encodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - A1 Insulator - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1K3_pBen-sfGFP_J23101-BenR

Plasmid

#128125

Purpose

Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

sfGFP and BenR

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBen and J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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C413-E22: attB4-polyhedrinp>-attB1r

Plasmid

#162922

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_XRCC4_XRCC4

Plasmid

#110047

Purpose

Protein expression and purification of XRCC4_XRCC4

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

XRCC4_XRCC4 (XRCC4 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-5'UTR(mtIF3)-mtIF3-Dendra2-3'UTR(mtIF3)

Plasmid

#182370

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

mtIF3 (Mtif3 Mouse)

Tags

Pal-Dendra2

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCK300

Plasmid

#87766

Purpose

empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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MTK2_008

Plasmid

#123703

Purpose

Encodes human pUbc as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

phUbC

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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actb2-lifeact-mKate2

Plasmid

#109750

Purpose

Ubiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2 fused to the actin binding lifeact sequence

Use

Zebrafish plasmids

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191184

Purpose

Expression of RPLP1 sensor in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:RPLP1.s1:EGFP-3xMS2

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)

Plasmid

#191175

Purpose

Mammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP (frameshifted)

Expression

Mammalian

Mutation

EGFP is frameshifted

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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274_pSKB3-Venus-LUMP

Plasmid

#65976

Purpose

Bacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensor

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Tags

Venus and thrombin site (11-residue linker that h…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pNB330

Plasmid

#60147

Purpose

Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Promoter

MET17pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

Plasmid

#164266

Purpose

sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6 promoter from W05B2.8

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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RCAN1_pCSdest

Plasmid

#53883

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RCAN1 (RCAN1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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HcKan_T

Plasmid

#65316

Purpose

receiving vector of terminators for the construction of standard terminator parts in YeastFab work

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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