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pEN_TT 3xFLAG Luciferase

Plasmid

#98391

Purpose

Gateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

Luciferase

Use

Entry vector for gateway cloning

Tags

3xFLAG

Promoter

TRE tight and TRE tight promoter

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJZC518

Plasmid

#62277

Purpose

Yeast dCas9 expression plasmid

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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CrBlue ---- pcDNA3.1

Plasmid

#62917

Purpose

CrBlue is a synthetic chimera comprising the blue opsin backbone with the intracellular loops and the C terminus of the jellyfish opsin, created to introduce spectral selectivity.

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

CrBlue

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only
pMCSG7-D16A-NmeCas9

Plasmid

#71476

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-D16A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

D16A

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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GST-N1IC

Plasmid

#47612

Purpose

bacterial expression of GST-myc-Notch1 IC

Depositor

Urban Lendahl

Article

Beatus et al Development. 1999 Sep;126(17):3925-35.

Insert

Notch1 intracellular domain (Notch1 Mouse)

Tags

GST and myc

Expression

Bacterial

Mutation

contains amino acids 1753-2531

Promoter

tac

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-mTUT7

Plasmid

#60044

Purpose

Mammalian expression of FLAG-tagged mouse TUT7

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT7 (Zcchc6 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern-mitochondria/pcDNA3

Plasmid

#51973

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to mitochondria

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

CoxVIIIx2

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-human MIZ1 wild type

Plasmid

#74161

Purpose

bacterial expression of MIZ1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 (ZBTB17 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
pCMV MN (eGFP)

Plasmid

#89932

Purpose

Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

MN

Tags

HA

Expression

Mammalian

Mutation

M.SssI residues 2-272 fragment

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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cacnb1b (rat) in pMT2 vector

Plasmid

#107423

Purpose

Expresses Calcium channel beta1b auxillary subunit in mammalian cells.

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 1997 Feb 15;17(4):1330-8.

Insert

calcium channel beta1b auxillary subunit

Expression

Mammalian

Promoter

Ad MLP

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMCSG7-H588A-NmeCas9

Plasmid

#71477

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-H588A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

H588A

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPR_DMS3g24

Plasmid

#89244

Purpose

Plasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

CRISPR

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shGFP

Plasmid

#83085

Purpose

Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pGAN213

Plasmid

#129270

Purpose

Control for measuring background levels of yTRAP reporter protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

mNeonGreen

Expression

Yeast

Promoter

min pCYC1

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMRXIP-Myc-YKT6

Plasmid

#116945

Purpose

Expressing Myc-YKT6 for mammalian cells

Depositor

Noboru Mizushima

Article

Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

Insert

Synaptobrevin homolog YKT6 (YKT6 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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Cdc2

Plasmid

#1886

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdc2 (CDK1 Human)

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
RCAS-myc

Plasmid

#11548

Depositor

Sandra Orsulic

Article

Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

Insert

c-myc (MYC Human)

Use

Retroviral

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pTK_BsmBI_LacZ_Cherry_Nanotag_117

Plasmid

#130552

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSplit-OC-U5-40K

Plasmid

#51741

Purpose

Express U5-40K-LucC fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

U5-40K (SNRNP40 Human)

Tags

FLAG and Luciferase (394-550aa)

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only
pCB6HindIII-rat FcRn

Plasmid

#121671

Purpose

Expresses rat neonatal Fc receptor in mammalian cells

Depositor

Pamela Bjorkman

Article

Tesar et al Traffic. 2006 Sep;7(9):1127-42.

Insert

rat neonatal Fc receptor (Fcgrt Rat)

Expression

Mammalian

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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