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MTK2_016

Plasmid

#123708

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-APIC-Flp1

Plasmid

#112806

Purpose

gateway cloning destination vector to express enhancer driven Flip1 in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-rbNHERF1

Plasmid

#31636

Depositor

Edward Weinman

Article

Voltz et al J Biol Chem. 2007 Nov 16. 282(46):33879-

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Tags

eGFP

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF70

Plasmid

#13193

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF70

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pMEP5-Flag Par6 WT

Plasmid

#24647

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

Par6 (Pard6a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pCKTRBS-OD

Plasmid

#102924

Purpose

pCK01 derivative carrying tetR under a constitutive promoter and an aTc-inducible PtetO promoter for control expression of genes. Contains LacI OD for translational fusions on the Ct of cloned genes.

Depositor

George Church

Article

Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

Type

Empty backbone

Expression

Bacterial

Promoter

PtetO

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y576F

Plasmid

#50506

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y576F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pICH44300

Plasmid

#50340

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, act2 (A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pNP1 tdTomato trigger

Plasmid

#107358

Purpose

Trigger sequence for pNP1 tdTomato toehold switch

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 5

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNP1 Aqua trigger

Plasmid

#107360

Purpose

Trigger sequence for pNP1 Aqua toehold switch

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 8

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNP1 OR input A

Plasmid

#107365

Purpose

Input A for pNP1 OR gate sfGFP

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate input A

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNP1 OR input B

Plasmid

#107366

Purpose

Input B for pNP1 OR gate sfGFP

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate input B

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFH88

Plasmid

#128201

Purpose

sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-Cre.ape

Plasmid

#71749

Purpose

U+36GFP-Cre fusion expression in E. Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)-Cre

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCK011

Plasmid

#105343

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK012

Plasmid

#105344

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-SEC7-Cerulean

Plasmid

#18851

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
CMVTO-Fyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp

Plasmid

#179668

Purpose

Membrane-bound (Fyn) TEVP-inducible caged-cTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged cTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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MTK4b_004

Plasmid

#123843

Purpose

Encodes spacer sequence as a Type 4b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Blast-cfCdc42_2

Plasmid

#26703

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Cdc42

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to canine Cdc42

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only