We narrowed to 29,602 results for: nans

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pcDNA3.1(+)/AUG-nLuc-3XFLAG

Plasmid

#127299

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pL-SFFV.Reporter.RFP657.PAC

Plasmid

#61395

Purpose

Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Plasmid

#92392

Purpose

produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Human Synapsin

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMSP1E3

Plasmid

#20064

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E3 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pET-15b-TEV-C9R

Plasmid

#118754

Purpose

TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

Depositor

Yutaka Kuroda

Article

Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

Insert

Tobacco Etch Virus protease (NEWENTRY Synthetic)

Tags

C9R and His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3A-BE5-NG-BlastR

Plasmid

#152998

Purpose

High purity C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA (short) wt

Plasmid

#19723

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

NOTE: short splice variant Long Sp. Variant: MV…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-LiMETER-v2

Plasmid

#113934

Purpose

Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

Depositor

Yubin Zhou

Article

He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

Insert

LOV2 and Stim1 polybasic domain (PB)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-GFP

Plasmid

#78546

Purpose

Fusion protein Cas9+GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSMART-Ala1

Plasmid

#65814

Purpose

NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Insulated waaHp from E. coli

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-mScarlet

Plasmid

#211330

Purpose

Mammalian overexpression vector for C-terminally mScarlet tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-GFP

Plasmid

#78548

Purpose

Cas9 protein with GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4-H6

Plasmid

#71717

Purpose

Membrane scaffold protein (MSP), helix 4 to 6 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85866

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-17-3p Sensor (MIR17 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-TAZ-N-Flag (S89A)

Plasmid

#19026

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin, S89A (site directed mu…

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pMXSIP-CMTM6-HA

Plasmid

#204710

Purpose

expresses CMTM6-HA in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

CMTM6 (CMTM6 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a hMDH2

Plasmid

#204230

Purpose

Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

hMDH2 (MDH2 Human)

Tags

6X His

Expression

Bacterial

Mutation

Does not include transit peptide and includes a M…

Promoter

Lac

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter hsa-let-7a-1

Plasmid

#46670

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-let-7a-1 (MIRLET7A1 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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PB-fLuc=tdT

Plasmid

#120870

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

pcDNA3-fLuc=tdT

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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