We narrowed to 10,984 results for: T7
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Plasmid#153297PurposeExpresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coliDepositorInsertGas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
TagsHis-tag and ssrA tagExpressionBacterialMutation1) N-terminal 6xHis tag flanked by 1x glycine res…PromoterT7Available SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
delNC C-His Ana GvpC in pET28a
Plasmid#85736PurposeExpresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coliDepositorInsertGas vesicle protein C (truncated and his-tagged)
TagsHis-tagExpressionBacterialMutationDeletion of N and C terminal regions, addition of…PromoterT7Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
Plasmid#167848PurposeBacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNADepositorInsertnsp10, nsp16
Tags6xHis and AviTagExpressionBacterialPromoterT7Available SinceApril 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
hSRAP.TWIN1.a13-69
Plasmid#49815Purposeexpression vector for SRAP(13-69) in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 13-69 (SRA1 Human)
Tagsintein & chitin binding domainExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXB4 (human) HIS-tag pET new RBS
Plasmid#8525DepositorInsertHOXB4 (HOXB4 Human)
TagsHis and T7ExpressionBacterialMutationContains improved Ribosomal binding site; makes …Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
p28BIOHTEV-LIC: nsp16_SARS-CoV-2
Plasmid#167846PurposeBacterial expression of the SARS-CoV-2 protein nsp16DepositorInsertnsp16
Tags6xHis and AviTagExpressionBacterialPromoterT7Available SinceApril 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-mCherry-mCherry-FLARE-EKAR-EV
Plasmid#123341PurposeRed-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.DepositorInsertmCherry-mCherry-FLARE-EKAR-EV
Tags6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet28a_RBPMS_1_129
Plasmid#65738Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
TagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-129PromoterT7Available SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-NRBF2 (1-159)
Plasmid#99331PurposeMammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complexDepositorInsertNuclear receptor-binding factor 2 (NRBF2 Human)
Tags6xHis-MBP-TEVExpressionBacterialMutationAA1-159 are presentPromoterT7Available SinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-msGC-NT21
Plasmid#78102Purposeexpresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and betaDepositorInsertsfragment of alfa subunit of sGC
fragment of beta subunit of sGC
TagsHis-tagExpressionBacterialMutationfragment 1-380 of the beta subunit and fragment 2…PromoterT7Available SinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-Drp1(deltaIB)
Plasmid#73041PurposeBacterial expression of mouse Drp1 isoform b (minus residues 514-602) with C-terminal His-tag and PreScission protease cleavage siteDepositorInsertDrp1(deltaIB) (Dnm1l Mouse)
Tags6xHis-tag and PreScission Protease siteExpressionBacterialMutationDeleted amino acids 514-602PromoterT7Available SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pet28a_RBPMS_1_100
Plasmid#65735Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
TagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-100PromoterT7Available SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
Plasmid#101208PurposeBacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variantDepositorInsertSpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S355C, S867C, N497A, R661A and Q695APromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph20-ETWV
Plasmid#133034PurposeExpresses Xph20-ETWV in bacteriaDepositorInsertXph20-ETWV
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAR480A-Q482A
Plasmid#128946Purposeexpresses R480A Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0003
Plasmid#42243PurposegRNA targeted to zebrafish gene fh [site #1]DepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AB (325-557, Δ 368-523)/pET28HMT
Plasmid#111052PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AB (325-557, Δ 368-523)PromoterT7Available SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ITPA_P32T
Plasmid#60825Purposeto purify human ITPA from bacteriaDepositorAvailable SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
M26I DJ-1 TEV site pET15b
Plasmid#60685PurposeRecombinant protein expression of Park7DepositorInsertM26I DJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationM26I and TEV site was inserted (see comments belo…PromoterT7Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-Amp
Plasmid#108704PurposeExpression of a His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsHis-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only