We narrowed to 15,153 results for: RING;

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pCI-neo VPS50-13Myc

Plasmid

#175093

Purpose

13Myc tagged VPS50 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

Insert

VPS50 (CCDC132 Human)

Tags

13Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pB-bsd-Flag-CNP

Plasmid

#126608

Purpose

Expresses N-terminal Flag-tagged human CNP

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

2'3' cyclic nucleotide phosphodiesterase (CNP Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CAG

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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amilGFP chromoprotein promoter-less

Plasmid

#118006

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, amilGFP

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-AD8-QES.i08.c04

Plasmid

#123259

Purpose

Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (AD8) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations P124D, …

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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CLPIT Cry2PHR-mCh-RhoA

Plasmid

#42959

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)

Use

Retroviral

Expression

Mammalian

Promoter

Tet-Off promoter

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Dppa2

Plasmid

#40799

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Dppa2 (Dppa2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159854

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pVenus-N-SLC35B2

Plasmid

#155038

Purpose

Transient expression of SLC35B2-Venus

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Tags

Venus

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-CyPet-Rac1

Plasmid

#24534

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (RAC1 Human)

Use

Retroviral

Tags

CyPet

Expression

Mammalian

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
YFP-Rac1(L61)

Plasmid

#11401

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Glutamine 61 mutated to Leucine.

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159811

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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optoPKAW196R/Y204A

Plasmid

#104546

Purpose

Expresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Cry2-mCherry-PKA W196R/Y204A

Tags

mCherry

Expression

Mammalian

Mutation

PKA W196R and Y204A

Promoter

CMV

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRGEB-Cas9-VirD2 (PCO)

Plasmid

#139802

Purpose

Expressing Cas9-VirD fusion under ubiquitin promoter

Depositor

Magdy Mahfouz

Article

Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

Insert

Cas9-VirD2

Tags

Cas9-VirD2

Expression

Plant

Promoter

Ubiquitin

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-pX330-Cas9-mCherry

Plasmid

#159742

Purpose

Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

Depositor

Stefan Kubicek

Article

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

donor plasmid targeting sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_B2515b

Plasmid

#91073

Purpose

Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

AtU6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTon1(acgfp1)

Plasmid

#233648

Purpose

Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.

Depositor

Takeo Kubo

Article

Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10

Insert

target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'

Use

Amphibian expression, tet-on

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTriEX-Antenna-GDI Cdc42

Plasmid

#101681

Purpose

"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Cdc42

Tags

6xHis and FRET Antenna (mCerulean-cpVenus)

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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MSP1594_2x_NLS

Plasmid

#110625

Purpose

Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
None

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-IRES-hrGFP-mWnt5a

Plasmid

#89772

Purpose

Expresses mouse Wnt5a in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Rodriguez et al J Neurosci. 2014 Jul 30;34(31):10285-97.

Insert

mouse Wnt5a (Wnt5a Mouse)

Use

AAV

Promoter

CMV

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRSpuro shID1#6

Plasmid

#19165

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 1 (ID1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only