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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 51,422 results for: des.1

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Showing: 9541 - 9560 of 51422 results

  6. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid
    #115694
    Purpose
    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer
    Article
    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:
    Inserts
    NmeCas9
    Nme-sgRNA
    Use
    CRISPR
    Tags
    NLS and NLS, HA
    Expression
    Insect and Mammalian
    Mutation
    None and fusion of repeat/tracrRNA to make a sgRNA
    Promoter
    EF-1 alpha and U6
    Available Since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    115694-plasmid-map-sequence-id-221986

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  7. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

    Plasmid
    #193769
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1ΔR198A/R210A (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193769-plasmid-map-sequence-id-380211

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  17. Cd99l2 long

    Plasmid
    #74469
    Purpose
    Expresses Cd99l2 transcript variant 1-IRES-EGFP under CAG promoter in mammalian cells
    Depositor
    Young Ho Suh
    Article
    Kang et al Cell Rep. 2025 Jan 28;44(1):115155. doi:
    Insert
    CD99 antigen-like 2, transcript variant 1 (Cd99l2 Mouse)
    Expression
    Mammalian
    Promoter
    chicken beta actin promoter
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 9541 - 9560 of 51422 results