We narrowed to 58,630 results for: plasmid
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Plasmid#87115PurposeSpCas9 expression AAV backbone plasmid under control of nEF promoterDepositorInsertnEF-Cas9
UseAAV and CRISPRTagsHA-NLSExpressionMammalianAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas
Plasmid#62225PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRDepositorHas ServiceCloning Grade DNAInsertcas9
UseCRISPR; Lambda red recombinaseExpressionBacterialPromoternative cas9 promoterAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40
Plasmid#100834PurposeAAV expression of ultrasensitive protein calcium sensor from CamKII promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCamKIIAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVax1-hVEGF165
Plasmid#74466Purposeexpression of hVEGF165 in mammalian cellsDepositorAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV Retrograde trial size)
Viral Prep#37825-AAVrg.TPurposeReady-to-use AAV Retrograde trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330A-nBRD4/PITCh
Plasmid#91794PurposePITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4DepositorInsertSpCas9
UseCRISPRExpressionMammalianPromoterchicken β-actin promoterAvailable SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-BbTagBY (AAV9)
Viral Prep#45185-AAV9PurposeReady-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsTagBFP, EYFP, or none.Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV1)
Viral Prep#51085-AAV1PurposeReady-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available SinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-roxSTOProx-ZsGreen
Plasmid#51274PurposeDre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreenDepositorInsertroxSTOProx-ZsGreen
ExpressionMammalianPromoterCAGAvailable SinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-H2B-AID-EYFP
Plasmid#47329Purposefor plasmid integration using the Flp-In system. AID at C-terminalDepositorAvailable SinceAug. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHsp70-Cas9
Plasmid#45945PurposeThe codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.DepositorInsertCodon optimized Cas9
UseCRISPRTags3x FLAGMutationSilent change from C to A at bp 834 of insertAvailable SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
myc-Rictor
Plasmid#1860PurposePlease see Addgene item #11367 for an updated version of this plasmid.DepositorAvailable SinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMXs-c-Myc
Plasmid#13375PurposeRetroviral expression of mouse c-MycDepositorAvailable SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hOCT3/4
Plasmid#17217PurposeRetroviral expression of human OCT3/4DepositorAvailable SinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEx-ER-TurboID
Plasmid#160857PurposeCre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorHas ServiceAAV8InsertER TurboID
UseAAVTagsHA and V5PromoterCAGAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV8)
Viral Prep#105545-AAV8PurposeReady-to-use AAV8 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHITagsEGFPAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (AAV1)
Viral Prep#100835-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (#100835). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-BbChT (AAV9)
Viral Prep#45186-AAV9PurposeReady-to-use AAV9 particles produced from AAV-EF1a-BbChT (#45186). In addition to the viral particles, you will also receive purified AAV-EF1a-BbChT plasmid DNA. Brainbow construct encoding mCherry and mTFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry, mTFP, or noneAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Oct3/4
Plasmid#13366PurposeRetroviral expression of mouse Oct3/4DepositorInsertPOU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
UseRetroviralExpressionMammalianMutationNoneAvailable SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV9)
Viral Prep#50476-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only