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pLKO.1-scrambled-GFP

Plasmid

#228736

Purpose

GFP expressing scramble of shRNA targeting Miro1

Depositor

Cagla Eroglu

Article

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Miro1 (Rhot1, Rhot1 Rat, Mouse)

Expression

Mammalian

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_R2_S170_LOV_P2.1_C3

Plasmid

#242155

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE P2.1.3 variant cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2 S170 LOV P2.1.3

Expression

Bacterial

Mutation

AraC: E3K, E144K, R146L, A152T, S225R, R251L; AsL…

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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HaloTag-SUMO1 Vector

Plasmid

#238699

Purpose

Express HaloTag-SUMO1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

SUMO1 (SUMO1 Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-eLaTranC

Plasmid

#246932

Purpose

for HEK293T human cell genome editing

Depositor

Caixia Gao

Article

Jin et al Cell. 2025 Sep 29:S0092-8674(25)01035-9.

Insert

eLaTranC

Use

CRISPR

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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HaloTag-SUMO3 Vector

Plasmid

#238697

Purpose

Express HaloTag-SUMO3 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

SUMO3 (SUMO3 Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLIC-His6-MBP-TEV-Rgs14

Plasmid

#236395

Purpose

Expresses His6-MBP-TEV-Rgs14 in E. coli

Depositor

John Hepler

Article

Brown et al J Biol Chem. 2015 Apr 3;290(14):9037-49.

Insert

Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)

Tags

hexahistidine (H6) tag and maltose-binding protei…

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-BmSc

Plasmid

#232208

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTRE3G-mouse cGAS-GFP

Plasmid

#186877

Purpose

Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Mouse cGAS with C-terminal GFP fusion (Cgas Mouse)

Use

Retroviral

Tags

GFP

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1-4

Plasmid

#218799

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1-4

Expression

Mammalian

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_ATG16L1:1-607

Plasmid

#211308

Purpose

Mammalian expression of full-length human ATG16L1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ATG16L1:M1-Y607 (ATG16L1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

T300A natural variant (VAR_021834)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196883

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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Pxyl-ftsZ-sfGFP

Plasmid

#174505

Purpose

Integrated plasmid harboring ftsZ-sfGFP gene, inducible by xylose (sequence of sfGFP optimized)

Depositor

Suliana Manley

Article

Mahecic et al Nat Methods. 2022 Sep 8. pii: 10.1038/s4

Insert

ftsZ

Tags

superfold GFP

Expression

Bacterial

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER

Plasmid

#123354

Purpose

Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

cp-moxCerulean3-moxCerulean3-FLARE-D1ER

Tags

C-terminal ER retention signal (KDEL), cp-moxCeru…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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JAM2_pCSdest

Plasmid

#53842

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

JAM2 (JAM2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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REEP5-mEmerald

Plasmid

#186964

Purpose

Express REEP5/DP1 with C-terminus mEmerald

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Reep5 (Reep5 Mouse)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC2A3

Plasmid

#132114

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A3 (SLC2A3 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV5 TBRI-His

Plasmid

#19161

Depositor

Joan Massague

Insert

transforming growth factor, beta receptor 1 (TGFBR1 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-LOV*-MVP

Plasmid

#202206

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to MVP in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Major vault protein (MVP Human)

Tags

FLAG and LOV*

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBUN6I11

Plasmid

#50580

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

dCas9-KRAB
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG, HA, and NLS

Mutation

dCas9 that is defective in DNA cleavage; the maiz…

Promoter

OsU3p and Ubi1p

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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