We narrowed to 56,967 results for: plasmid
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Plasmid#113712PurposeSingle pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.DepositorInsert12GLI-FLuc
UseMule gateway entry vectorExpressionMammalianAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS
Plasmid#158058PurposeDonor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable markerDepositorInsertsglmS homologous region 1
glmS homologous region 2 + MCS3
UseSynthetic BiologyAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
YSBE 610 : pHYG_6xHA
Plasmid#92091PurposePlasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selectionDepositorInsert6xHA
Usetagging proteins in cryptococcus neoformanAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
YSBE 516 : pNEO_4xFLAG
Plasmid#92082PurposePlasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, Neo selectionDepositorInsert4xFlag
Usetagging proteins in cryptococcus neoformanAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
YSBE 731 : pHYG_4xFLAG
Plasmid#92090PurposePlasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, HYG selectionDepositorInsert4xFlag
Usetagging proteins in cryptococcus neoformanAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDNR-gRNA
Plasmid#206990PurposeA plasmid for expression of SaCas9-sgRNA targeting donor plasmidsDepositorInsertDonor plasmid-targeting SaCas9-gRNA
ExpressionMammalianPromoterU6Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
YSBE 517 : pNEO_6xHA
Plasmid#92083PurposePlasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, Neo selectionDepositorInsert6xHA
Usetagging proteins in cryptococcus neoformanAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
YSBE 559 : pNAT_6xHA
Plasmid#92087PurposePlasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, NAT selectionDepositorInsert6xHA
Usetagging proteins in cryptococcus neoformanAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
YSBE 558 : pNAT_4xFLAG
Plasmid#92086PurposePlasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, NAT selectionDepositorInsert4xFlag
Usetagging proteins in cryptococcus neoformanAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIS003
Plasmid#194014PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertC16 Ex4 XTR f1
ExpressionBacterialAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEDA5_GFPmut3_Y66H
Plasmid#80085PurposePositive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.DepositorInsertGFPmut3_Y66H
Tags6x-His tagExpressionBacterialMutationchanged Tyrosine 66 to HistidinePromoterproBAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pM2s2AsG
Plasmid#137924PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDCC6
Plasmid#59985PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassetteDepositorInsertsCas9
U6-2>sgRNA
UseCRISPRTags3xFLAGPromoterU6-96Ab and hsp70BbAvailable SinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJAT7
Plasmid#204292PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.DepositorInsert3XP3-DsRed
UseCRISPRPromoter3XP3Available SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xAP-1_RE::Renilla
Plasmid#178317PurposeAP-1 Renilla luciferase reporterDepositorInsertAP-1 Renilla Reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN-Sth1Cas9
Plasmid#221386PurposeCas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attBDepositorInsertSth1 Cas9
UseCRISPRAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pN3FS-CYC1
Plasmid#85783PurposeN-ICE plasmid pH3FS-CYC1DepositorInsertloxP-NatMX-loxP-CYC1p-3FLAG
UseCre/LoxExpressionYeastMutation*(see below)Available SinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
2xP53_RE::FLuc
Plasmid#178316PurposeP53 Firefly luciferase reporterDepositorInsertP53 FLuc Reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMT-flp
Plasmid#67274PurposeConjugally transferable Flp recombinase plasmid for unmarked mutagenesisDepositorInserttetA, flp, repA101, oriR101
UseFlp recombinase plasmid, temperature sensitiveTagsHis-SUMO (flp)PromotertetA promoter of plasmid pACYC184 originAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVH607-Tier1-PCREm4-Nluc
Plasmid#169594PurposeTier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).DepositorInsertcAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4Available SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only