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mEos3.2-Clathrin-15

Plasmid

#57452

Purpose

Localization: Clathrin Vesicles, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Article

Huang et al Nat Methods. 2013 Jul;10(7):653-8. doi:

Insert

Clathrin (CLTB Human)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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psicheck2 PTEN 3'UTR

Plasmid

#50936

Purpose

reporter for PTEN RNAi

Depositor

Pier Pandolfi

Article

Tay et al Cell. 2011 Oct 14;147(2):344-57. doi: 10

Insert

PTEN 3'UTR (PTEN Human)

Use

RNAi

Tags

Renilla luciferase

Expression

Mammalian

Promoter

T7

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pET28b-ptetO::bac::gfp

Plasmid

#217882

Purpose

expresses the bac-BGC in bacterial cells, produces bacillamide D

Depositor

Tobias A. M. Gulder

Article

Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

Inserts

Dehydrogenase
Non-ribosomal peptide synthetase
Aminotransferase

Expression

Bacterial

Promoter

ptetO

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-β2AR-μDpTT

Plasmid

#182326

Purpose

Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

beta2AR-μDronpa-TagRFP-T (ADRB2 Human)

Tags

Full-length human beta2 adrenergic receptor

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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LPAR4-DuET

Plasmid

#213331

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

LPAR4 (LPAR4 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-TfR

Plasmid

#12130

Depositor

Pamela Bjorkman

Article

Lebron et al Cell. 1998 Apr 3. 93(1):111-23.

Insert

transferrin receptor (TFRC Human)

Tags

6X-His tag, Factor Xa cleavage site, and leader p…

Expression

Insect

Mutation

The portion of the human TfR gene encoding residu…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
p2R3a-VenusYFP-3AT

Plasmid

#71269

Purpose

Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

VenusYFP

Use

Gateway Cloning

Tags

4xGly linker and T3A pea Pisum sativum ribulose-1…

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

Plasmid

#118274

Purpose

Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

bPAC(WT)
SthK

Use

AAV

Tags

His tag, P2A, mCherry, and myc tag

Expression

Mammalian

Promoter

CamK II

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTetON-BMP2/7

Plasmid

#137913

Purpose

Inducible mammalian co-expression vector for human bone morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, divergent)

Depositor

Georg Feichtinger

Article

Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

Inserts

BMP2 (BMP2 Human)
BMP7 (BMP7 Human)

Expression

Mammalian

Promoter

TRE

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLCO2A1

Plasmid

#131949

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO2A1 (SLCO2A1 Human)

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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AURKA_HUMAN_D0

Plasmid

#79739

Purpose

This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

AURKA (AURKA Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-syn-FLEX-synaptoPAC-minWPRE

Plasmid

#153100

Purpose

Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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NEK1_HUMAN_D0

Plasmid

#79725

Purpose

This plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK1 (NEK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MERTK_HUMAN_D0

Plasmid

#79705

Purpose

This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MERTK (MERTK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MARK3_HUMAN_D0

Plasmid

#79740

Purpose

This plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MARK3 (MARK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCS2plus-mVenus1-155-I152L-GGGS

Plasmid

#162613

Purpose

Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Expression

Mammalian

Mutation

1-155-I152L

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIIα-μDpTT

Plasmid

#182327

Purpose

PKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

RIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)

Tags

Full-length human PKA type 2alpha regulatory subu…

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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EPHB1_HUMAN_D0

Plasmid

#79694

Purpose

This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB1 (EPHB1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK3_HUMAN_D0

Plasmid

#79689

Purpose

This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK3 (STK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
GAK_HUMAN_D0

Plasmid

#79682

Purpose

This plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

GAK (GAK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only