We narrowed to 90,026 results for: Mal

1. pCMV_RBMX-eA3A-UdgX-HF-nCas9

   Plasmid

   #163548

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-HF-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-dCas9-HA

   Plasmid

   #61355

   Purpose

   encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

   Plasmid

   #205631

   Purpose

   Expression of TnpBmax in mammalian cells

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   ISDra2-TnpB optimized for mammalian expression
   

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LAMP1-mRFP-FLAG

   Plasmid

   #34611

   Purpose

   3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

   Depositor

   David Sabatini

   Article

   Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   2xFlag and mRFP

   Expression

   Mammalian

   Available Since

   March 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA

   Plasmid

   #108747

   Purpose

   Expresses N-terminus to aa27 of Flp recombinase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment N-terminus to aa27
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

   Plasmid

   #108770

   Purpose

   Expresses aa397 to C-terminus of Flp recombinase fused to PYL CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment aa397 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and PYL

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-lyso-pHluorin

   Plasmid

   #70113

   Purpose

   Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   lyso-pHluorin (Cd63 Aequorea victoria, Mouse)
   

   Tags

   HA-Tag and pHluorin

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-Tet-OsTIR1(F74G)-V5

   Plasmid

   #158664

   Purpose

   Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   Tet-OsTIR1(F74G) AAVS1
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   F74G

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MC4R

   Plasmid

   #233630

   Purpose

   encodes the human melanocortin 4 receptor, without any tags

   Depositor

   Christian Gruber

   Article

   Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

   Insert

   MC4R (MC4R Human)
   

   Expression

   Mammalian

   Promoter

   CMV/T7

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. FH-TET1-pEF

    Plasmid

    #49792

    Purpose

    This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

    Depositor

    Anjana Rao

    Article

    Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

    Insert

    Tet methylcytosine dioxygenase 1 (TET1 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

    Plasmid

    #134868

    Purpose

    Expresses EGFP-tagged lysosomal marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc

    Plasmid

    #21850

    Depositor

    David Ron

    Article

    Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

    Insert

    ATF4 (Atf4 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    ATF4 5' UTR and AUG fused to Luciferase driv…

    Available Since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xFLAG-dCas9/pCMV-7.1

    Plasmid

    #47948

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A (catalyticall…

    Promoter

    CMV

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-EFS-EGFP-T2A-Bsd

    Plasmid

    #193198

    Purpose

    Lentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance marker

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PEmaxRNaseHtrunc

    Plasmid

    #207858

    Purpose

    Mammalian expression of PEmaxDRNaseH prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PEmaxDRNaseH
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ1658-dCas9-EGFP

    Plasmid

    #51023

    Purpose

    Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    dCas9 fuse to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    MSCV LTR promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-GRP78/BiP

    Plasmid

    #32701

    Depositor

    Richard C. Austin

    Article

    Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

    Insert

    GRP78/BiP (HSPA5 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEos3-Rac1

    Plasmid

    #92039

    Purpose

    Expresses mEos3-Rac1 fusion protein

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEos3-dnRac1

    Plasmid

    #92040

    Purpose

    Expresses mEos3 fusion with dnRac1 (T17N).

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T17N (dominant negative)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEos3-caRac1

    Plasmid

    #92041

    Purpose

    Expresses mEos3 fusion with caRac1 (Q61L)

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L (constitutively active)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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