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pPOTv7-blast-Ppyr9eh

Plasmid

#201443

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

Ppyr9eh

Use

Unspecified

Promoter

read through

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG:Ppyr9eh

Plasmid

#201441

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG:Ppyr9eh

Use

Unspecified

Promoter

read through

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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Atg20-24 pST39

Plasmid

#200439

Purpose

His6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Inserts

Atg20
Atg24

Tags

6xHis-TEV

Expression

Bacterial

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-puro-3Ty:mScarletI:3Ty

Plasmid

#201093

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-hygro-3Ty:mScarletI:3Ty

Plasmid

#201092

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-His:mScarletI:His

Plasmid

#201098

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-His:mNG:His

Plasmid

#201095

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-3Ty:mScarletI:3Ty

Plasmid

#201091

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-hygro-3Ty:tagRFPt:3Ty

Plasmid

#201088

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-g418-3Ty:tagRFPt:3Ty

Plasmid

#201087

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-blast-3Ty:tagRFPt:3Ty

Plasmid

#201086

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-g418-tagRFPt

Plasmid

#201085

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-hygro-tagRFPt

Plasmid

#201084

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0023_T2c_BCD_1.5K

Plasmid

#200053

Purpose

Bicistronic RBS 1.5K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 1.5K

Expression

Bacterial

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_175

Plasmid

#199107

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

Dummy ORI plus NGS barcode 23, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_172

Plasmid

#199104

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSG5 ORI plus NGS barcode 20, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0027_T2c_BCD_13K

Plasmid

#200049

Purpose

Bicistronic RBS 13K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 13K

Use

Synthetic Biology

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0025_T2c_BCD_4.6K

Plasmid

#200051

Purpose

Bicistronic RBS 4.6K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 4.6K

Use

Synthetic Biology

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0024_T2c_BCD_2.3K

Plasmid

#200052

Purpose

Bicistronic RBS 2.3K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 2.3K

Use

Synthetic Biology

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0022_T2c_BCD_0.75K

Plasmid

#200054

Purpose

Bicistronic RBS 0.75K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.75K

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge