169,450 results
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Plasmid#124167PurposepiggyBac-based expression of rtTA-M2DepositorInsertrtTAM2-IRES-Hph
ExpressionMammalianPromoterCAGAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-CDK12-V5-TurboID_IDG-K
Plasmid#135244PurposeGateway destination clone of CDK12 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCDK12-V5-TurboID (CDK12 Human)
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianPromoterUbiquitinAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIGFP_W220F
Plasmid#46772PurposeProtein overexpression with the modified LacIWF repressorDepositorInsertLacI_W220F (lacI )
MutationTryptophan 220 replaced by PhenylalanineAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Human V5-IRF3-pcDNA3
Plasmid#32713DepositorAvailable SinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-ABE8e(V106W)
Plasmid#198533PurposeExpresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSUN5
Plasmid#25491PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15b His-p97
Plasmid#169020PurposeExpresses His-p97 in bacteriaDepositorInsertp97
TagsHisExpressionBacterialPromoterT7Available SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA
Plasmid#176743PurposeExpresses Connexin-43 fused with BioID2 at the C-terminus in astrocytesDepositorInsertCx43-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-RH▲-MNase
Plasmid#136292PurposeExpression of catalytic inactive RNase H fused to micrococcal nucleaseDepositorInsertRNase HI-D10R-E48R and MNase
ExpressionBacterialAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 human RNF146 WWE (99-183)
Plasmid#132613Purposeexpresses human RNF146 WWE (99-183) in E. coliDepositorInsertRNF146 WWE (99-183)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDZ617 pKAN 12xPP7 V4
Plasmid#72237PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert12xPP7 V4
UseCre/LoxExpressionBacterialAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-eYFP (AAV Retrograde)
Viral Prep#117382-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagseYFPAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HAPLN1_WT
Plasmid#81757PurposeGateway Donor vector containing HAPLN1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human Lipid Droplet and Metabolism Library
Pooled Library#191535PurposeTargets 1,196 genes related to lipid droplet biology and cell metabolism.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS-FLAG-cGAS
Plasmid#127659PurposeOver-express EGFP-NLS-FLAG-cGASDepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP2371
Plasmid#127577PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSDC803_IKZF1_mutant
Plasmid#224113PurposeInsect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blottingDepositorInsertIKZF1 (IKZF1 Human)
TagsFlag-TEVExpressionInsectMutationResidues 140-196, Q146A and G151NPromoterpolyhedrinAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2424
Plasmid#142421PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 GFP B55 alpha shRNA 3-1
Plasmid#10679DepositorAvailable SinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMEH343 CD63 MS2 HA
Plasmid#168217PurposeMammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tagDepositorAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only