We narrowed to 10,361 results for: POR
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pCR3.1-CREM2 luc
Plasmid#106460Purposeluciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)DepositorInsertluciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)
UseLuciferaseExpressionMammalianMutationCRE site adjacent to NFkB site mutatedAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc C1A4E
Plasmid#141088Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of NanolucDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E
ExpressionMammalianMutationPlease see depositor commentsPromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-OgNLuc
Plasmid#135951PurposeExpresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorInsertOgNLuc LumiFluor
TagsT2 mitochondrial localization sequenceExpressionMammalianPromoterEF1aAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
A3H HapII R175/6E-Cas9n-UGI
Plasmid#119142PurposeBase editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EEDepositorInsertAPOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI
UseCRISPRMutationA3H Hap II RR175/6EEPromoterCMVAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_R89A-PolyA
Plasmid#112291PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with Arginine 89 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_L91A-PolyA
Plasmid#112292PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with Leucine 91 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_F95A-PolyA
Plasmid#112293PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with Phenylalanine 95 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP
Plasmid#120908PurposeExpresses Ptch1-B (V111FL114FW115A)DepositorInsertPtch1 (Ptch1 Mouse)
TagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP
Plasmid#120907PurposeExpresses Ptch1-B (I766FV111F)DepositorInsertPtch1 (Ptch1 Mouse)
TagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-Sfxn2 (CG6812)
Plasmid#110643PurposeRetroviral expression of Flag-tagged Drosophila CG6812 in mammalian cellsDepositorInsertCG6812 (CG6812 Fly)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)Available SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS Exon
Plasmid#110119PurposeExpression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicingDepositorAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-J5TNY4
Plasmid#103116PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertJ5TNY4(Cas9 coding gene from Porphyromonas sp. oral taxon 279 str. F0450)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15T+1X
Plasmid#108188PurposePCNA1-PdX fusion proteinDepositorInsertPCNA1-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationC73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G)
Plasmid#107855PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationG368W and W385G in splicing reporterAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)Y815A
Plasmid#100744PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutantDepositorAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup88-XbaI
Plasmid#87328PurposeTo express shRNA against human Nup88DepositorInsertshRNA against human Nup88
UseRNAiExpressionMammalianPromoterH1Available SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-prRDH-CTS
Plasmid#86069PurposeA bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.DepositorInsertRetinol dehydrogenase (RDH8 Bovine)
TagsGSTExpressionBacterialMutationthe insert is 297-312 of RDH8 (Bos taurus)PromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-cystin-CTS
Plasmid#86066PurposeA bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.DepositorInsertCystin (Cys1 Mouse)
TagsGSTExpressionBacterialMutationcontains the region from 28-35PromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only
