We narrowed to 152,634 results for: Des

1. pGGDestTol2LC-4sgRNA

   Plasmid

   #64242

   Purpose

   destination vector for four U6x:sgRNA cassettes

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST17-UbE2B

   Plasmid

   #15781

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2B (UBE2B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pRL055 pDESTattB_cryaa:Venus

   Plasmid

   #213509

   Purpose

   attB-containing pDEST plasmid, for use in Multisite Gateway reaction

   Depositor

   Christian Mosimann

   Article

   Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSDest-HA-TMPRSS2

   Plasmid

   #154963

   Purpose

   TMPRSS2 with N-terminal HA

   Depositor

   Utpal Pajvani

   Article

   Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

   Insert

   HA-TMPRSS2 (TMPRSS2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pXNubA22-Dest

   Plasmid

   #105087

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

   Depositor

   Christopher Grefen

   Article

   Grefen et al Methods Mol Biol. 2009;479:217-33. doi:

   Type

   Empty backbone

   Use

   Tags

   NubA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST17-UbE2F

   Plasmid

   #15800

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2F (UBE2F Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pFRT-TODestRFP_RNAseH1

   Plasmid

   #65785

   Purpose

   expresses RFP-tagged RNAseH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Human, Zebrafish)
   

   Use

   Tags

   RFP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDestattB/CY (pCM327)

   Plasmid

   #68341

   Purpose

   Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.

   Depositor

   Christian Mosimann, Leonard Zon

   Article

   Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

   Type

   Empty backbone

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pATT-Dest

   Plasmid

   #79770

   Purpose

   Staging plasmid for use in DIP transposition

   Depositor

   David Savage

   Article

   Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

   Type

   Empty backbone

   Use

   Synthetic Biology; Staging plasmid for transposit…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVTU-Dest

    Plasmid

    #105088

    Purpose

    Yeast expression vector for Gateway cloning and SUS bridge assays

    Depositor

    Christopher Grefen

    Article

    Grefen et al Methods Mol Biol. 2009;479:217-33. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFRT-TODestFLAGHA_RNAseH1

    Plasmid

    #65782

    Purpose

    expresses FLAGHA-tagged RNAsH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Human, Zebrafish)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-SAM-Dest

    Plasmid

    #102563

    Purpose

    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    N/A

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGGDestTol2LC-2sgRNA

    Plasmid

    #64240

    Purpose

    destination vector for two U6x:sgRNA cassettes

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 2HA-pDEST-N

    Plasmid

    #118373

    Purpose

    Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-SorLA-v2

    Plasmid

    #154900

    Purpose

    Expresses SorLA fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    SORL1 (SORL1 Human)
    

    Use

    Tags

    Venus fragment 2 (v2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST_6xUAS:mNeonGreen-Rab5a

    Plasmid

    #192355

    Purpose

    zfRabaa tagged with mNeonGreen, expressed under the UAS promoter

    Depositor

    Francesca Peri

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    Rab5a (rab5aa Zebrafish)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDest490-OPN-a

    Plasmid

    #17590

    Depositor

    Xin Wang

    Article

    Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

    Insert

    OPN-a (SPP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Base pairs 49–942

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pDEST51-LRRK2-WT

    Plasmid

    #25080

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-Desmoglein2-N-18

    Plasmid

    #55032

    Purpose

    Localization: Desmosomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Desmoglein2 (DSG2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pFRT-TODestRFP_HuD

    Plasmid

    #65766

    Purpose

    expresses RFP-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V23M mutation in RFP

    Promoter

    CMV, 2x Tet

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only