Addgene

We narrowed to 9,742 results for: otos

Showing: 1441 - 1470 of 9742 results

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pCXLE-dCas9VP192-T2A-EGFP

Plasmid

#69536

Purpose

Episomal plasmid encoding dCas9VP192

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-EGFP

Use

CRISPR

Tags

EGFP

Expression

Mutation

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETa_ mEos4b

Plasmid

#51073

Purpose

Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric)

Depositor

Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

mEos4b

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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eMagA-EGFP-Mito

Plasmid

#162244

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagA-EGFP-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-1

Plasmid

#13003

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 (SULF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-NbALFA-degFusionRed

Plasmid

#199466

Purpose

Expression of the photodegradable protein degFusionRed fused to an anti-ALFA-tag-specific nanobody in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

NbALFA-degFusionRed

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pPAmCherry1-N1

Plasmid

#31928

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Use

Tags

Expression

Mammalian

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

cmv

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-PhoCl2f-mCherry

Plasmid

#164048

Purpose

Optogenetic control of protein localization by PhoCl2f

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2f-mCherry

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-VP3SA11

Plasmid

#89164

Purpose

Express Rotavirus SA11 VP3 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP3

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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RSRF-Luc-2wt

Plasmid

#31818

Depositor

Astar Winoto

Article

Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Insert

MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

MEF2-dependent (-307 to -242 of Nur77/NR4A1)

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pT7-NSP4SA11

Plasmid

#89171

Purpose

Express Rotavirus SA11 NSP4 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP4

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-VP4SA11

Plasmid

#89165

Purpose

Express Rotavirus SA11 VP4 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP4

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-NSP3SA11

Plasmid

#89170

Purpose

Express Rotavirus SA11 NSP3 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP3

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330S-3

Plasmid

#58779

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-NSP1SA11

Plasmid

#89168

Purpose

Express Rotavirus SA11 NSP1 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-NSP5SA11

Plasmid

#89172

Purpose

Express Rotavirus SA11 NSP5 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP5

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-NSP2SA11

Plasmid

#89169

Purpose

Express Rotavirus SA11 NSP2 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-VP6SA11

Plasmid

#89166

Purpose

Express Rotavirus SA11 VP6 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP6

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-VP7SA11

Plasmid

#89167

Purpose

Express Rotavirus SA11 VP7 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP7

Use

Tags

*see below

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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eMagAF-EGFP-Mito

Plasmid

#162243

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagAF-EGFP-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAmCherry1-C1

Plasmid

#31929

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Use

Tags

Expression

Mammalian

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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LentiV_Blast

Plasmid

#111887

Purpose

Lentiviral expression of cDNA with blasticidin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-2

Plasmid

#13004

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 2 (SULF2 Human)

Use

Tags

6x His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-PhoCl2c

Plasmid

#164035

Purpose

Improved PhoCl variant with enhanced dissociation efficiency

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

PhoCl2c

Use

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Mutation

Promoter

araBad promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET-PhoCl1-6His

Plasmid

#164033

Purpose

Expressed PhoCl1 in a pET expression vector

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

PhoCl1

Use

Tags

6xHis Tag

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/Myc-His(-)-MSulf-2

Plasmid

#13008

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

mouse sulfatase 2 (Sulf2 Mouse)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-PhoCl2f

Plasmid

#164036

Purpose

Improved PhoCl variant with faster dissociation rate

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

PhoCl2f

Use

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Mutation

Promoter

araBad promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330S-4

Plasmid

#58780

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A-1x3

Plasmid

#58767

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiV_Puro

Plasmid

#111886

Purpose

Lentiviral expression of cDNA with puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET FLIPE-600n

Plasmid

#13537

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge