Addgene

We narrowed to 16,658 results for: ef1

Showing: 961 - 980 of 16658 results

Results per page:

Sort results by:

EF1a-TET1-dCas9

Plasmid

#235593

Purpose

Tet1CD fused to N-terminus of dCas9

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)

Use

CRISPR

Tags

3xNLS, TET1 CD, and tagBFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-dCas9-VP64

Plasmid

#235595

Purpose

Expresses the dCas9-VP64 under the control of human EF1a promoter

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

dCas9-VP64

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-CFAPorig

Plasmid

#221092

Purpose

FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS314-Cef1-M175I

Plasmid

#234118

Purpose

Suppressor screen mutant in Cef2

Depositor

Aaron Hoskins

Insert

Cef1

Use

Tags

Expression

Yeast

Mutation

M175I

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS314-Cef1-Q193P

Plasmid

#234117

Purpose

Suppressor screen mutant in Cef1

Depositor

Aaron Hoskins

Insert

Cef1

Use

Tags

Expression

Yeast

Mutation

Q193P

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS314-Cef1-A37V

Plasmid

#234119

Purpose

Suppressor screen mutant in Cef3

Depositor

Aaron Hoskins

Insert

Cef1

Use

Tags

Expression

Yeast

Mutation

A37V

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#233538

Purpose

A CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-NFAPoptim

Plasmid

#221088

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-CFAPoptim

Plasmid

#221089

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-NFAPorig

Plasmid

#221091

Purpose

FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-NFAPoptim

Plasmid

#221093

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-CFAPoptim

Plasmid

#221094

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-NFAPorig

Plasmid

#221086

Purpose

FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-CFAPorig

Plasmid

#221087

Purpose

FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-hfCas13d-pa

Plasmid

#233037

Purpose

To express hfCas13d from a EF1a promoter

Depositor

Jonathan Ploski

Insert

hfCas13d

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 EF1a-EGFP

Plasmid

#231925

Purpose

3rd generation lentiviral transfer plasmid with EGFP reporter gene

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#232123

Purpose

A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Gateway cloning

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAdeasy-CMV-AE-EF1α-R2

Plasmid

#194928

Purpose

expressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosis

Depositor

Limei Wang

Article

Wang et al Vaccines (Basel). 2024 Sep 6;12(9):1022.

Inserts

AE
EEF1A1 (EEF1A1 Human)
R2

Use

Adenoviral

Tags

Ag85B-ESAT6, EF1a, and Rv2031c-Rv2626c

Expression

Mutation

a 48 bp linker (Gly3ser)4 was added between the t…

Promoter

CMV and EF1a

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_CdTag-HA

Plasmid

#221493

Purpose

NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge