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pAAV-nEF-Con/Foff 2.0-iC++-EYFP

Plasmid

#137156

Purpose

Intersectional viral expression of ic++-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-iC++-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual-M2

Plasmid

#135584

Purpose

High eukaryotic dual protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Article

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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3HA-ERHBD-Hoxb8

Plasmid

#212932

Purpose

Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

Depositor

Hans Haecker

Article

Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

Insert

3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Mouse, Human)

Use

Retroviral

Tags

Triple HA tag

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-PPO-Venus (AAV9)

Viral Prep

#139505-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Promoter

EF1a

Tags

Venus (Cre-dependent)

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only
Human DNA Binding Domain-Focused CRISPR Knockout Library

Pooled Library

#123334

Purpose

Designed for genomic deletion screening of transcription factors by targeting the DNA binding domains.

Depositor

Christopher Vakoc

Article

Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only
pGP-CMV-jGCaMP7c variant 1513

Plasmid

#105320

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pDNA2.0 6H-TEV-KRAS

Plasmid

#111849

Purpose

Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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p-AAV-sh[control]

Plasmid

#75438

Purpose

control shRNA vector

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

GFP

Use

AAV and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-hLIN28A

Plasmid

#47902

Purpose

Expresses human LIN28A in mammalian cells using retrovirus system.

Depositor

Shinya Yamanaka

Article

Tanabe et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

human LIN28A (LIN28A Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pAJ075_hsCRBN

Plasmid

#124214

Purpose

Insect cell expression vector for His6 tagged hsCRBN

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

Cereblon (CRBN Human)

Tags

His6-TEV

Expression

Insect

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pHelper_ShCAST

Plasmid

#127922

Purpose

Expresses ShCAST and a crRNA targeting PSP1

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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L4437 x6TRE (cJun and AP-1) Ben DsRed-Express2 in TUPV1

Plasmid

#244197

Purpose

Expression of a DsRed-Express2 reporter under a cJun-responsive promoter (AP-1 binding sites)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

cJun responsive promoter (6 AP-1 binding sites + YB_TATA), dsRedExpress2 reporter

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SomaGCaMP6f2 (AAV9)

Viral Prep

#158757-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Promoter

CAG promoter

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-mNeonGreen

Plasmid

#99134

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRCC-K

Plasmid

#81191

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-ChR2-mCherry

Plasmid

#137144

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-sRGECO

Plasmid

#137127

Purpose

Intersectional viral expression of sRGECO in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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