We narrowed to 10,808 results for: T7
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Plasmid#101184PurposeHuman expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A)
Tags3x FLAG and NLS (SV40)MutationG582A/V583A/E584A/D585A/N588A/Q926APromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW2993 - human expression plasmid for SpCas9 Cluster 2
Plasmid#101185PurposeHuman expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
Tags3x FLAG and NLS (SV40)ExpressionMammalianMutationG582A/V583A/E584A/D585A/N588APromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A
Plasmid#101179PurposeHuman expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster1+Q926A(N692A/M694A/Q695A/H698A/Q926A)
Tags3x FLAG and NLS (SV40)ExpressionMammalianMutationN692A/M694A/Q695A/H698A/Q926APromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A
Plasmid#101180PurposeHuman expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
Tags3x FLAG and NLS (SV40)ExpressionMammalianMutationN692A/M694A/Q695A/Q926APromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
FN(9-10, RGE)-I27-CC
Plasmid#85396PurposeMTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertFibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
Tags6x HisExpressionBacterialMutationGRGDS motif mutated to GRGESPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO-BROX 1-411; K141E
Plasmid#89865PurposeExpresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299DepositorInsertBROX (BROX Human)
TagsHis Tag, V5 tagExpressionBacterialMutationmutated lysine 141 to glutamic acidPromoterT7Available SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO-BROX 1-411; L212D
Plasmid#89864PurposeExpresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300DepositorInsertBROX (BROX Human)
TagsHis Tag, V5 tagExpressionBacterialMutationmutated leucine 212 to aspartic acidPromoterT7Available SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
KS6A1_HUMAN_D1
Plasmid#79720PurposeThis plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1G3_HUMAN_D0
Plasmid#79685PurposeThis plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)
Plasmid#61935PurposeExpression of hRPN13 (285-407) (Q337A/Q338A) in E.coliDepositorInserthRPN13 (285-407) (ADRM1 Human)
TagsGSTExpressionBacterialMutationConstruct starts at residue 285, residues Q337 an…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS
Plasmid#61940PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coliDepositorTags6x HISExpressionBacterialMutationConstruct ends at residue 156, residues 97-100 (N…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)
Plasmid#61941PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coliDepositorTags6x HISExpressionBacterialMutationConstruct ends at residue 117, stop codon introdu…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL4D-V5-His6
Plasmid#67426PurposeExpression of hArl4D with C-term V5 and His6 tag in E. coliDepositorInsertARL4D (ARL4D Human)
TagsHis and V5ExpressionBacterialMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterT7/lacOAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet28a_RBPMS_1_120
Plasmid#65737Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
TagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-120PromoterT7Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet28a_RBPMS_1_144
Plasmid#65739Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
TagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-144PromoterT7Available SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet23a_RBPMS_20_196
Plasmid#65734Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
TagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 20-196PromoterT7Available SinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet23a_RBPMS_1_129
Plasmid#65731Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
TagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-129PromoterT7Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet23a_RBPMS_1_111
Plasmid#65729Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
TagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-111PromoterT7Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet28a_RBPMS_20_196
Plasmid#65741Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
TagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 20-196PromoterT7Available SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR™221_RBPMS2
Plasmid#59389PurposepDONR vector with RBPMS2DepositorInsertRBPMS2 (RBPMS2 Human)
PromoterT7Available SinceFeb. 10, 2015AvailabilityAcademic Institutions and Nonprofits only