We narrowed to 14,362 results for: T7
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Plasmid#85624PurposeBacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)DepositorInsert10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Schistosoma japonicum, Synthetic, Human)
Tags10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA)
Plasmid#192442PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.DepositorInsertmCherry-TSC2(S939A,T1439A) (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+36)-DL4
Plasmid#199168Purposeused to express a supercharged GFP variant that forms inclusion bodies in E. coliDepositorInsertGFP(+36)-DL4
TagsMGHHHHHHGGAMutationE16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…PromoterT7Available SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_StefinA
Plasmid#182402PurposeBacterial expression of stefinADepositorAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-mTDGa.1
Plasmid#81051Purposebacterial expression of catalytically dead murine TDGDepositorInsertTDG (Tdg Mouse)
Tags6HISExpressionBacterialMutationasparagine 151 changed to alaninePromoterT7Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII
Plasmid#209292PurposeExpresses R2Bm-Cas9(H840A) fusion in E. coliDepositorInsertR2
TagsHis, MBP, SpCas9(H840A), StrepII, and bdSUMOExpressionBacterialPromoterT7Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETa(+) HpCsd3 (42-403)
Plasmid#71285PurposeExpresses Csd3 from Helicobacter pyloriDepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
p4979 pcDNA4-E6AP III L502P
Plasmid#32892DepositorInsertE6AP (UBE3A Human)
Tags6xHis Express and T7-tagExpressionMammalianMutationL502PPromoterCMVAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
p4978 pcDNA4-E6AP III E550L
Plasmid#32891DepositorInsertE6AP (UBE3A Human)
Tags6xHis Express and T7-tagExpressionMammalianMutationE550LPromoterCMVAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCA528-AMOT(1-300)-sortase
Plasmid#166706Purposeexpresses AMOT(1-300) with C-terminal sortase tag in bacterial cellsDepositorInsertAMOT aa 1-300
TagsHis-SUMO and Sortase tagExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
CNNM3-CBS-YPet
Plasmid#149686PurposeFor the FRET-based binding assay between human CNNM3 CBS domain and PRL2.DepositorInsertCNNM3-CBS-YPet (CNNM3 Synthetic, Human)
Tags8 histidine tagExpressionBacterialPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)
Plasmid#117908PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain
Plasmid#101205PurposeBacterial expression plasmid for SpCas9 REC3 domainDepositorInsertSpCas9 variant K506–Q712
Tags10x His, MBP, and TEV siteExpressionBacterialMutationK506–Q712PromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
p4973 pcDNA4-E6AP III C21Y
Plasmid#32890DepositorInsertE6AP (UBE3A Human)
Tags6 His Express and T7-tagExpressionMammalianMutationC21YPromoterCMVAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMtb CblA
Plasmid#138298PurposeExpression of Mtb CblADepositorInsertMtb CblA
TagsHis tagExpressionBacterialPromoterT7Available SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-synPCB2.0
Plasmid#139477PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
ExpressionMammalianMutationV413LPromoterCAG promoterAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-EPA-DNNNS-DQNRT
Plasmid#128397PurposeIn vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sitesDepositorInsertExotoxin A
Tags6xHis tagExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 FOXM
Plasmid#67118PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1-GATA4-opposite orientation
Plasmid#51928Purposefor T7 directed antisense production of mouse GATA4DepositorAvailable SinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C, A92E)
Plasmid#79046PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C, A92E)
ExpressionBacterialMutationA14C, E45C, A92E mutations in HIV-1 CA proteinPromoterT7Available SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only