We narrowed to 18,505 results for: eomes

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GFP-RhoA

Plasmid

#23224

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoA (RHOA Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pLNCX2-mEGFP-CHMP2A

Plasmid

#115328

Purpose

expresses mEGFP-CHMP2A in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2A (CHMP2A Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Plasmid

#175777

Purpose

pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TMEM192 (TMEM192 Human)

Use

CRISPR

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP-VP16

Plasmid

#183923

Purpose

VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP-VP16

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15516_BEAR-GFP-2in1

Plasmid

#174082

Purpose

BEAR-GFP plasmid with a gRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA

Plasmid

#176745

Purpose

Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Glt1-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-Turbo-L2

Plasmid

#127350

Purpose

gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pIND(V5)EcoDam

Plasmid

#59201

Purpose

Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pRluc-LC3wt

Plasmid

#105002

Purpose

Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCAT005

Plasmid

#171632

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-B2M
mNeon

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1-a and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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C1A-C1A-EYFP

Plasmid

#61155

Purpose

Diacylglycerol (DAG) reporter

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)

Tags

EYFP

Expression

Mammalian

Mutation

C1A from PKCG amino acids 26-89

Promoter

CMV

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-TYR

Plasmid

#32781

Depositor

Ruth Halaban

Article

Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

Insert

Tyrosinase (TYR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AktAR(T/A)

Plasmid

#118463

Purpose

Negative-control mutant for ExRai-AktAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-GNB1

Plasmid

#133856

Purpose

Mammalian expression of GNB1 N-terminally tagged with EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

GNB1 (GNB1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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V5-bArr2

Plasmid

#201493

Purpose

Expression of the beta-arrestin2 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

V5-bArr2 (ARRB2 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Rab5

Plasmid

#174454

Purpose

Express EGFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp38-C1

Plasmid

#134548

Purpose

Expresses YFP-Rpp38 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp38 (RPP38 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42-wt

Plasmid

#12975

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo1-G3BP1_pTRE-G418

Plasmid

#202015

Purpose

expresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-LOV-Turbo1-G3BP1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

pTRE-Tight

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pKSEe401R

Plasmid

#218532

Purpose

CRISPR/Cas9 genome editing vector pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone.

Depositor

Kirill Demchenko

Article

Kiryushkin et al Int J Mol Sci. 2024 Feb 6;25(4):1975. do

Type

Empty backbone

Expression

Plant

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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