167,948 results

1. pLenti PGK GFP Blast (w510-5)

   Plasmid

   #19069

   Purpose

   3rd gen lentiviral eGFP expression vector, PGK promoter, Blast

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CP2-TCC

   Plasmid

   #179679

   Purpose

   Complementary plasmid

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [sd2] T7 RNAP-TCC linker-degron
   

   Expression

   Bacterial

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZT-AAVS1-R1

   Plasmid

   #52638

   Purpose

   TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

   Depositor

   Jizhong Zou, Mahendra Rao

   Article

   Luo et al Stem Cells Transl Med. 2014 May 15. pii:

   Insert

   Right AAVS1 TALEN
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCRISPRa-v2, Gene Expression (m5), top 5 sgRNAs/gene

   Pooled Library

   #84002

   Purpose

   Mouse CRISPRa Pooled Library targeting gene expression

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Available Since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pLOX-TERT-iresTK

   Plasmid

   #12245

   Purpose

   2nd generation lentiviral vector for reversible immortalization of human primary cells

   Depositor

   Didier Trono

   Article

   Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

   Insert

   TERT, HSV1-TK (TERT Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   LoxP in 3' LTR.

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 MCS-BirA(R118G)-HA

   Plasmid

   #36047

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

   Depositor

   Kyle Roux

   Article

   Roux et al J Cell Biol. 2012 Mar 12.

   Has Service

   Cloning Grade DNA

   Insert

   BirA(R118G)-HA
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Available Since

   April 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-PSDΔ1,2-FRBx3

   Plasmid

   #225827

   Purpose

   A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

   Depositor

   Haruo Kasai

   Article

   Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

   Insert

   PSDΔ1,2-FRBx3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGT-Ah10

   Plasmid

   #122577

   Purpose

   MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   tetQ/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

   Plasmid

   #199349

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-SUMOEu

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZT-AAVS1-L1

    Plasmid

    #52637

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Jizhong Zou, Mahendra Rao

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Left AAVS1 TALEN
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHIV-Luciferase

    Plasmid

    #21375

    Purpose

    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. human MMP-9 promoter

    Plasmid

    #53434

    Purpose

    promoter activity assay

    Depositor

    Douglas Boyd, Etty Benveniste

    Article

    Ma et al J Immunol. 2001 Nov 1;167(9):5150-9.

    Insert

    MMP-9 promoter (MMP9 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    MMP9

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid

    #61362

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL4.23-GW

    Plasmid

    #60323

    Purpose

    This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

    Plasmid

    #183775

    Purpose

    AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    NLS-GFP
    

    Use

    AAV

    Mutation

    NA

    Promoter

    CAG

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-Streptavidin-Glutamate_Tag

    Plasmid

    #46367

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Glutamate_Tag
    

    Tags

    6 glutamate tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCIP-Flag-TfebAA

    Plasmid

    #79014

    Purpose

    For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Tfeb (Tfeb Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S142A, S211A

    Promoter

    pCAGGs

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1_V5-TWIST1

    Plasmid

    #215030

    Purpose

    Mammalian expression of human TWIST1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    twist family bHLH transcription factor 1 (TWIST1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PGKp-GFP-YAP (5SA)

    Plasmid

    #174174

    Purpose

    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Five LATS phosphorylation sites (S61, S109, S127,…

    Promoter

    PGK

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pcDNA3-FLAG-Luciferase

    Plasmid

    #58792

    Purpose

    Express FLAG-tagged Luciferase in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    firefly Luciferase
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only