We narrowed to 211 results for: LPP

1. pDule-IBBN (G2)

   Plasmid

   #85500

   Purpose

   Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

   Insert

   IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32G L65E F108W Q109M D158S R162K

   Promoter

   lpp (constitutive)

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDule2-pCNF

   Plasmid

   #85495

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDule-pCNF

   Plasmid

   #85494

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDule-3-nitroTyrosine (5B)

   Plasmid

   #85498

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32H H70C D158S I159A L162R

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDule2-3-nitroTyrosine (5B)

   Plasmid

   #85499

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32H H70C D158S I159A L162R

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDule2-para-aminoPhe

   Plasmid

   #85503

   Purpose

   Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

   Insert

   p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32T, E107T, D158P, I159L, L162A

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDule-para-aminoPhe

   Plasmid

   #85502

   Purpose

   Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

   Insert

   p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32T, E107T, D158P, I159L, L162A

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDule2-Tet2.0

   Plasmid

   #85497

   Purpose

   Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

   Insert

   Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32G L65Q F108S Q109D D158S L162N

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDule-Tet2.0

   Plasmid

   #85496

   Purpose

   Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

   Insert

   Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32G L65Q F108S Q109D D158S L162N

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDule2-IBBN (G2)

    Plasmid

    #85501

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDule-IBBN (G2)

    Plasmid

    #85500

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKS044

    Plasmid

    #65472

    Purpose

    pTrc plasmid expressing clpP

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    clpP (clpP )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDule-3-nitroTyrosine (5B)

    Plasmid

    #85498

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

    Plasmid

    #174515

    Purpose

    Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Inserts

    Mm PylS
    MmtRNA-Pyl-opt(UGA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ASL from the serT gene (UGA anticodon) and recoded

    Promoter

    GlnS and lpp

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDule2-3-nitroTyrosine (5B)

    Plasmid

    #85499

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-SEAP

    Plasmid

    #24595

    Depositor

    Alan Cochrane

    Insert

    SEAP (ALPP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDule2 - Mj Acd A9

    Plasmid

    #197652

    Purpose

    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

    Depositor

    Ryan Mehl

    Article

    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

    Inserts

    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32A, L65D, F108L, Q109E, D158S, L162T

    Promoter

    GlnS and lpp

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDule- Mj Acd A9

    Plasmid

    #197651

    Purpose

    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

    Depositor

    Ryan Mehl

    Article

    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

    Inserts

    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32A, L65D, F108L, Q109E, D158S, L162T

    Promoter

    GlnS and lpp

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pERM2-nhpSer

    Plasmid

    #201922

    Purpose

    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    E412P, E414F, T417K, P495M, I496W, F529S and H67R…

    Promoter

    GlnS (constitutive), OXB20 (constitutive), lpp (c…

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pALS2-sfGFP WT

    Plasmid

    #197575

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    M. alvus Pyl-tRNA (6)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    araC and lpp

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pALS2-sfGFP 150TAG

    Plasmid

    #197574

    Purpose

    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    araC and lpp

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQhsCDtRNA+

    Plasmid

    #86014

    Purpose

    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene

    Depositor

    Hong Li

    Article

    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

    Inserts

    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    

    Use

    Co-expression; purification

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    amino acids 1-401, amino acids 1-411, and amino a…

    Promoter

    LPP and Ptac

    Available since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pClp-Wasabi

    Plasmid

    #86108

    Purpose

    mWasabi reporter gene driven by ClpP/ClpX promoter

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Insert

    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pClpP/ClpX

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLK65

    Plasmid

    #37257

    Depositor

    Ann Hubbard

    Article

    Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

    Insert

    vang-like 2 (VANGL2 Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pQC-SEAP #M004#725

    Plasmid

    #42125

    Depositor

    Ayano Satoh

    Article

    Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

    Insert

    alkaline phosphatase, placental (SEAP) (ALPP Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pTECH-chPylRS(IPYE)

    Plasmid

    #99222

    Purpose

    Expression plasmid encoding chPylRS(IPYE) and pylT

    Depositor

    Dieter Söll

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chPyl(IPYE)RS; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTECH-chAcK3RS(IPYE)

    Plasmid

    #104069

    Purpose

    Expression plasmid encoding chAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTECH-MmPylRS(IPYE)

    Plasmid

    #104073

    Purpose

    Expression plasmid encoding MmPylRS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp MmPylRS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTECH-MbAcK3RS(IPYE)

    Plasmid

    #104070

    Purpose

    Expression plasmid encoding MbAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp MbAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTECH-MmAcK3RS(IPYE)

    Plasmid

    #104071

    Purpose

    Expression plasmid encoding MmAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp MmAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge